福建农业学报2025，Vol.40Issue(11)：1132-1139,8.DOI:10.19303/j.issn.1008-0384.2025.11.005
海水酸化对褐牙鲆呼吸代谢的影响
Respiratory Metabolism of Paralichthys olivaceus Affected by Seawater Acidification
摘要
Abstract
[Objective]Effects of seawater acidification on respiratory metabolism and related gene expressions of Paralichthys olivaceus were studied,so as to provide scientific theoretical basis for the conservation of P.olivaceus resources in an increasingly acidified marine environment.[Method]In enclosed still water tanks,P.olivaceus weighed(2.16±0.34)g were raised for 49 d with water at normal pH at 8.10 as control and at pH 7.90,7.70,7.50,and 7.30 adjusted by CO2 infusion for treatments.Oxygen consumption and ammonia excretion of the fish were measured,and expressions of abcg 8 and glulb monitored using qRT-PCR.[Result]The seawater pH had significant effects on the oxygen consumption rate and ammonia excretion rate,and the gene expression of metabolic genes abcg 8 and glulb(P＜0.05).As the pH of seawater was lowered in the tank,the rates of oxygen consumption and ammonia excretion,O:N ratio,as well as the expressions of abcg 8 and glulb of P.olivaceus rose significantly(P＜0.05).By the end of 49 d,the fish in the water of pH 8.10(CK),7.90,7.70,7.50,and 7.30 had average oxygen consumption rates of 1.10,1.24,1.44,1.58,and 1.54 mg·g-1·h-1,average ammonia excretion rates of 0.23,0.23,0.25,0.25,0.26 mg·g-1·h-1,and O:N ratio of 4.70,4.98,5.03,5.78,and 5.39,respectively.Seawater acidification at pH 7.30-7.50 has a significant effect on respiratory metabolism and metabolism-related genes of P.olivaceus.[Conclusion]The respiratory metabolism and abcg 8 and glulb expressions of P.olivaceus in a tank were significant affected by the acidified seawater in the range of pH 7.30 to pH 7.50.关键词
海水酸化/褐牙鲆/呼吸代谢/基因表达Key words
seawater acidification/Paralichthys olivaceus/respiratory metabolism/gene expression分类
农业科技引用本文复制引用
薛佳欣,常一凡,庄诗雨,吴天木,张泽林,温锦,秦玉雪,尹增强,陈雷..海水酸化对褐牙鲆呼吸代谢的影响[J].福建农业学报,2025,40(11):1132-1139,8.基金项目
辽宁省教育厅基本科学研究面上项目(JYTMS20230491) （JYTMS20230491）
辽宁省教育厅科学研究经费项目(JL202001) （JL202001）
农业农村部渔业渔政管理局政府购买服务项目(09240202、09240071) （09240202、09240071）
