海洋渔业2025，Vol.47Issue(6)：737-746,10.
中国东南沿海不同地理群体锈斑蟳遗传多样性分析
Genetic diversity analysis of different geographic populations of Charybdis feriata along the coast of southeast China
摘要
Abstract
Charybdis feriata belongs to Arthropoda,Crustacea,Portunidae,and Charybdis,and is one of the large aquatic economic crabs in China.To investigate the genetic diversity and genetic differentiation of different geographic populations of Charybdis feriata along the coast of southeast China,13 pairs of microsatellite marker primers were used to analyze the genetic diversity and genetic structure characteristics of a total of 150 samples from five geographic populations:Sanya(HN)in Hainan,Beihai(GX)in Guangxi,Yangjiang(GD)in Guangdong,Zhangzhou(FJ)in Fujian and Ningbo(ZJ)in Zhejiang.The observed number of alleles(Na)of the 13 microsatellite loci ranged from 5 to 31,and the average effective number of alleles(Ne)was 8.190±3.064.The average polymorphism information content(PIC)of the 13 loci was 0.477-0.909,with high polymorphism.The gene flow(Nm)of the 13 microsatellite loci in the five populations of Charybdis feriata ranged from 12.164 to 31.467,with a mean value of 19.016±1.628.A total of three loci(XBX007,XBX122,and XBX153)conformed to the Hardy-Weinberg equilibrium(P<0.05),while the remaining 10 loci significantly deviated from the Hardy-Weinberg equilibrium.The mean values of the effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He)and Shannon's index(I)of the five populations of Charybdis feriata were 6.794±2.494-8.193±2.654,0.704±0.139-0.762±0.157,0.822±0.095-0.853±0.090 and 2.126±0.417-2.242±0.434,indicating that the five geographic populations showed high genetic diversity.AMOVA showed that the genetic variation was mainly from within individuals(85%),and only a small portion of the variation was between individuals within the populations(15%),and that the genetic variation was mainly present at the individual level.The results of Nei's genetic distance showed that the average genetic distance between populations was 0.106-0.128,with the smallest genetic distance in HN and GD populations,and the largest in HN and FJ populations.The genetic differentiation coefficients(Fst)between the five geographic populations were 0.006-0.010,indicating that the degree of genetic differentiation between all populations was low(0<Fst<0.05).All individuals of Charybdis feriata did not show obvious geographic clustering,and could be regarded as a geographic unit as a whole.The study shows that there is no significant geographic structure or genealogical structure among the five geographic populations of Charybdis feriata along the southeast coast of China.The overall genetic diversity is high,and the results can provide basic information and scientific guidance for the subsequent evaluation of the germplasm resources and the sustainable utilization of Charybdis feriata.关键词
锈斑蟳/微卫星/遗传多样性/遗传结构/东南沿海Key words
Charybdis feriata/microsatellite/genetic diversity/genetic structure/southeast China coast分类
农业科技引用本文复制引用
唐棣,杨明秋,邹雄,刘洪涛..中国东南沿海不同地理群体锈斑蟳遗传多样性分析[J].海洋渔业,2025,47(6):737-746,10.基金项目
海南省重点研发项目(ZDYF2021XDNY278) （ZDYF2021XDNY278）
2024年海南省科技条件平台专项(重点实验室)建设项目 （重点实验室）
