海洋渔业2025，Vol.47Issue(6)：783-795,13.
基于转录组解析低温和饥饿对大黄鱼生理功能的影响
Effects of cold and fasting on the physiological functions of Larimichthys crocea based on transcriptomic analysis
摘要
Abstract
Large yellow croaker Larimichthys crocea is a main commercial marine fish,and its output is the highest among sea cage-cultured fishes in China.To investigate the effects of low temperature and fasting on gene expressions in the liver of L.crocea,samples with a body mass of(21.38±2.46)g were divided into four groups:control group(C group),cold group(CC group),fasting group(F group)and cold+fasting group(CF group),3 replicates in each treatment.The liver samples were sequenced by Illumina Hiseq 2500.The results showed that a total of 2 981,3 483,1 987,2 591 and 3 516 differentially expressed genes(DEGs)were obtained from CC vs.C,CF vs.F,F vs.C,CF vs.CC and CF vs.C,respectively.The DEGs were significantly enriched in four important common pathways:glutathione metabolism,cytokine-cytokine receptor interaction,TCA cycle and PPAR signaling pathway,indicating antioxidant defense,inflammatory reaction,aerobic metabolism and lipid metabolism played important roles in the adaptation of fish to low temperature or(and)starvation.In addition,the DEGs of CC vs.C and CF vs.F were enriched in unsaturated fatty acid biosynthesis,indicating that fish could improve tolerance to cold stress by changing cell membrane fluidity.The DEGs of F vs.C and CF vs.CC were enriched in primary bile acid biosynthesis,indicating that fish could improve fasting tolerance by promoting lipid digestion and absorption.Thus,the overwintering strategy of L.crocea involved signaling pathways such as antioxidant,inflammatory response,aerobic metabolism,and lipid metabolism.The results provide a scientific basis for further study about the effects of overwintering on the physiological functions of L.crocea.关键词
大黄鱼/低温/饥饿/转录组学Key words
Larimichthys crocea/cold stress/fasting/transcriptome分类
农业科技引用本文复制引用
吉群,宋炜,张成林,谢正丽,李莉珍,徐豪,韩旭磊,黄少聪..基于转录组解析低温和饥饿对大黄鱼生理功能的影响[J].海洋渔业,2025,47(6):783-795,13.基金项目
国家自然科学基金(42476111) （42476111）
国家重点研发计划(2022YFD2401102 （）
2023YFC2812105) （）
青岛海洋科学与技术试点国家实验室山东省专项经费(2022QNLM30001) （2022QNLM30001）
中国水产科学研究院基本科研业务费(2020TD76) （2020TD76）
