井冈山大学学报（自然科学版）2026，Vol.47Issue(1)：44-51,8.DOI:10.3969/j.issn.1674-8085.2026.01.006
不同地龙蛋白酶解液体外抗氧化活性比较研究
Comparative study on the antioxidant activity in vitro of different earthworm protein hydrolysates
摘要
Abstract
In this study,the earthworm protein hydrolysates were prepared through enzymatic hydrolysis,and the antioxidant activity in vitro of the hydrolysates produced by different proteases was compared and analyzed.Earthworm protein was first extracted via alkali extraction and acid precipitation.Subsequently,five hydrolysates were generated using pepsin,trypsin,papain,neutral protease,and alkaline protease.The BCA protein assay measured the hydrolysate concentrations.Three antioxidant methods in vitro(DPPH radical scavenging,ABTS radical scavenging,and Fe3+reducing power assay)were used to evaluate the antioxidant effects.The experimental results show that the yield of earthworm protein hydrolysates for the alkali-extracted acid-precipitated earthworm protein is 5.57 g(11.14％).All five hydrolysates have the antioxidant activity in vitro.Papain hydrolysate exhibites the strongest antioxidant capacity in both DPPH radical scavenging and Fe3+reducing power tests.Neutral protease hydrolysate show the highest activity in ABTS radical scavenging.This study compared the antioxidant effects in vitro of the enzymatic hydrolysates of earthworm protein obtained through different proteases,which can provide a reference for the development and utilization of earthworm protein resources.关键词
地龙/碱提酸沉法/蛋白/酶解液/体外抗氧化活性Key words
earthworm/alkali-acid precipitation/protein/hydrolysate/in vitro antioxidant activity分类
化学化工引用本文复制引用
罗海怡,倪林君,邓静文,罗辉,余丽双..不同地龙蛋白酶解液体外抗氧化活性比较研究[J].井冈山大学学报（自然科学版）,2026,47(1):44-51,8.基金项目
国家自然科学基金项目(82074005) （82074005）
福建省科技厅计划项目(2024J01130,2024Y9510) （2024J01130,2024Y9510）
江西省自然科学基金面上项目(20252BAC240486) （20252BAC240486）
