江西农业大学学报2025，Vol.47Issue(6)：1472-1481,10.DOI:10.3724/aauj.2025127
山药不同繁殖材料苗期根际土壤微生物群落多样性分析
Analysis of microflora diversity in inter-root soil of different propagating materials of yam at seedling stage
摘要
Abstract
[Objective]This study aims to investigate the characteristics of rhizosphere soil microflora communities during the seedling stage of different propagating materials of yam,thus providing a scientific basis for the healthy cultivation of yam and the selection of propagation materials.[Method]The characteristics of microbial community structure of rhizosphere soil in tuber head,tuber section and bulbil of yam were compared by pot method and high-throughput sequencing technology.[Result]Alpha diversity analysis showed that soil bacterial and fungal species richness and diversity were significantly higher in bulbil than in tuber head.Principal coordinate analysis(PCoA)showed that the tuber were more similar to the bulbil in bacterial community structure,and the tuber head were more similar to the tuber section in fungal community structure.Analysis of bacterial community composition revealed that tuber head were significantly enriched with Cyanobacteria and the genus norank_f_norank_o_Chloroplast,while tuber section and bulbil showed significant enrichment of Chloroflexi and Acidobacteriota.Fungal community analysis demonstrated that both tuber head and tuber section were significantly enriched with the potential pathogenic genus Talaromyces,whereas bulbil were notably enriched with beneficial fungal genera Mortierella and Chaetomium.Community differentiation analysis indicated significant differences in both bacterial and fungal community structures between tuber head versus tuber section/bulbil,while tuber section and bulbil exhibited high similarity in microbial community composition.[Conclusion]This study demonstrates that different yam propagation materials significantly influence rhizosphere soil microbial communities,thereby altering the microecological environment for yam growth.Cultivation using stem segment and bulbil can enhance microbial diversity and improve soil health.Bulbil planting can enrich beneficial fungi and enhance systemic disease resistance.However,tuber head cultivation poses a risk of pathogenic fungal enrichment.These findings provide a microbiological basis for healthy yam cultivation practices.关键词
山药/不同繁殖材料/根际/土壤微生物Key words
yam/different propagating materials/rhizosphere/soil microbe分类
农业科技引用本文复制引用
周佳,黄渝岚,申章佑,劳承英,周灵芝,李艳英,李素平..山药不同繁殖材料苗期根际土壤微生物群落多样性分析[J].江西农业大学学报,2025,47(6):1472-1481,10.基金项目
广西自然科学基金项目(2023GXNSFBA026290)Project supported by the the Natural Science Foundation of Guangxi(2023GXNSFBA026290) 南宁市科学研究与技术开发计划项目(20232055)、广西农业科学院基本科研业务专项(桂农科2023YM78、桂农科2024YP057)和广西农科院团队项目(桂农科2021YT056)同时对本研究给予了资助,谨致谢意! （2023GXNSFBA026290）
