江西农业大学学报2025，Vol.47Issue(6)：1530-1539,10.DOI:10.3724/aauj.2025132
出芽短梗霉菌PA-2对藜叶片光合特性和活性氧积累的影响
Effects of Aureobasidium pullulans PA-2 on photosynthetic characteristics and reactive oxygen species accumulation of Chenopodium album L.leaves
摘要
Abstract
[Objective]To explore the response characteristics of Chenopodium album leaves to the fermentation broth of Aureobasidium pullulans PA-2 strain and provide a theoretical basis for the control of C.album by PA-2.[Method]Taking C.album as the research object,pot experiment was conducted to measure and analyze the photosynthetic characteristics and reactive oxygen species(ROS)accumulation of C.album leaves after spraying strain PA-2 fermentation broth(treatment)and sterile water(control).[Result]The results showed that,compared with control,the chlorophyll content of C.album leaves in the treatment group decreased significantly,and net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)showed an overall downward trend after the treatment of strain PA-2,while the intercellular CO2 concentration(Ci)increased significantly,indicating that photosynthetic inhibition was mainly dominated by non-stomatal limiting factors.The dynamic changes of quantum yield of regulated energy dissipation[Y(NPQ)],non-regulated energy dissipation[Y(NO)]and non-photochemical quenching(NPQ)were the most significant,indicating that the fluorescence index was more sensitive to PA-2 treatment.The content of O2·⁻ and H2O2 in the leaves of treatment group was significantly higher than that of control,and it showed obvious enrichment in the leaves on the 7th day.[Conclusion]Strain PA-2 fermentation broth could inhibit the activity of photosynthetic electron transport chain and destroy the homeostasis of antioxidant system,resulting in excessive accumulation of ROS and failure of photosynthetic assimilation ability,and ultimately causing physiological damage to the leaves of C.album.关键词
出芽短梗霉菌PA-2/藜/叶绿素含量/光合参数/叶绿素荧光参数/活性氧Key words
Aureobasidium pullulans PA-2/Chenopodium album L./chlorophyll content/photosynthetic parameters/chlorophyll fluorescence parameters/active oxygen分类
农业科技引用本文复制引用
莫姗姗,李娟,王信,朱海霞,程亮..出芽短梗霉菌PA-2对藜叶片光合特性和活性氧积累的影响[J].江西农业大学学报,2025,47(6):1530-1539,10.基金项目
青海省自然科学基金项目(2022-ZJ-911)Project supported by Qinghai Provincial Natural Science Foundation Project(2022-ZJ-911) （2022-ZJ-911）
