江苏农业学报2025，Vol.41Issue(12)：2315-2325,11.DOI:10.3969/j.issn.1000-4440.2025.12.004
促生抗旱微生物对烟草抗旱性的调控作用
Drought-resistance regulatory mechanism of plant growth-promoting and drought-tolerant microorganisms in tobacco under drought stress
摘要
Abstract
In order to alleviate the effects of drought stress on the growth of tobacco,non-antagonistic growth-promo-ting and drought-resistant microorganisms from the rhizosphere soil of drought-stressed tobacco were screened and identified in this study,and sterile water was used as control(CK),the effects of drought-resistant microorganisms on physiological and biochemical indices of tobacco and soil microbial community structure under drought stress were analyzed by root irriga-tion experiments with single and compound drought-resistant microorganisms.The results showed that a total of seven strains with the ability to dissolve phosphorus,pro-duce indoleacetic acid(IAA),produce siderophore and produce 1-aminocyclopropane-1-carboxylic acid(ACC)deaminase were identified.Among them,strain L5N1 had the strongest growth-promoting ability,and strain S2-1 had the strongest drought resistance.L5N1 and S2-1 were iden-tified as Kluyvera intermedia and Pseudomonas lini respectively.The growth-promoting and drought-resistant microorganisms could improve the agronomic traits of tobacco,reduce the malondialdehyde content of tobacco leaves,and improve the an-tioxidant capacity of tobacco.Among them,the combination of strains L5N1 and S2-1 had the most significant effect.After 21 days of drought stress,the soil moisture content of L5N1 and S2-1 combined treatment was 63.20％higher than that of CK,and the soil enzyme activities of sucrase,dehydrogenase,urease and acid phosphatase were 457.14％,416.91％,53.54％and 93.80％higher than those of CK,respectively.The relative abundance of Pseudomonas,Gemmata and Acidibacter was significantly higher than that of CK.In summary,the combination of the growth-promoting strain L5N1 and the drought-resistant strain S2-1 screened in this study can effectively improve the growth performance and adaptability of tobacco under drought stress.关键词
干旱胁迫/烟草/促生抗旱微生物/植株抗旱性/土壤酶活Key words
drought stress/tobacco/growth-promoting and drought-resistant microorganisms/plant drought resist-ance/soil enzyme activity分类
农业科技引用本文复制引用
唐茂翔,李斌,闫芳芳,李鹏,李建鹏,韩勤,高永翔,刘东阳,邱忠平..促生抗旱微生物对烟草抗旱性的调控作用[J].江苏农业学报,2025,41(12):2315-2325,11.基金项目
国家自然科学基金项目(52570179) （52570179）
中国烟草总公司四川省公司科技专项(SCYC202409) （SCYC202409）
西南交通大学博士创新项目(CX2025YB17) （CX2025YB17）
