集成技术2026，Vol.15Issue(1)：67-74,8.DOI:10.12146/j.issn.2095-3135.20250911001
人偏肺病毒快速检测方法的建立及应用
Establishment and Application of a Rapid Detection Method for Human Metapneumovirus
毛茅 1吴峰 2江坤 2万晓春3
作者信息
- 1. 中国科学院深圳先进技术研究院 生物医药与技术研究所 蛋白与细胞药物研究中心 深圳 518055||深圳市光明区科技创新服务中心 深圳 518107
- 2. 深圳市药品检验研究院 深圳 518057
- 3. 中国科学院深圳先进技术研究院 生物医药与技术研究所 蛋白与细胞药物研究中心 深圳 518055
- 折叠
摘要
Abstract
Currently,the detection of human metapneumovirus(hMPV)primarily relies on methods such as viral nucleic acid testing,viral antigen detection,viral culture and isolation,and serological testing.These methods are often complex,time-consuming,costly,and highly dependent on specialized equipment.In contrast,lateral flow immunochromatography offers advantages such as low cost,high specificity,strong stability,rapid detection,and the ability to be performed without specialized personnel or equipment,providing a new approach for hMPV detection.This study utilized monoclonal antibody technology to obtain specific monoclonal antibodies targeting the N protein of hMPV.The antibodies were used to label colloidal gold and applied for the detection of human metapneumovirus N protein based on a double-antibody sandwich immunochromatographic assay.The established rapid detection method for human metapneumovirus N protein achieved a detectio limit of 0.1 μg/L,demonstrating high sensitivity,accuracy,and specificity.This method can be used for rapid on-site detection of metapneumovirus.关键词
人偏肺病毒/N蛋白/单克隆抗体/免疫检测Key words
human metapneumovirus/N protein/monoclonal antibodies/immunoassay分类
医药卫生引用本文复制引用
毛茅,吴峰,江坤,万晓春..人偏肺病毒快速检测方法的建立及应用[J].集成技术,2026,15(1):67-74,8.
