江苏农业学报2025，Vol.41Issue(12)：2334-2340,7.DOI:10.3969/j.issn.1000-4440.2025.12.006
稻纵卷叶螟FOXO调控Cu/Zn SOD参与抗杆状病毒CnmeGV感染的机制
Mechanism by which Cnaphalocrocis medinalis FOXO regulates Cu/Zn SOD to mediate resistance to CnmeGV infection
摘要
Abstract
To elucidate the resistance mechanism of Cnaphalocrocis medinalis to C.medinalis granulovirus(CnmeGV),this study analyzed the response mechanism of transcription factor FOXO and copper/zinc superoxide dismutase(Cu/Zn SOD)in C.medinalis to viral infection.The results showed that the expression level of Cu/Zn SOD in fat body of C.medinalis larvae was higher than those in hemolymph,midgut and Malpighian tube.At 6-48 h after CnmeGV infection,the activity of superoxide dismutase in the fat body of C.medinalis increased significantly,and the mRNA expression levels of FOXO and Cu/Zn SOD genes were significantly up-regulated,and the antioxidant N-acetylcysteine(NAC)could inhibit the up-regulation.Both FOXO inhibitor AS1842856 and SOD inhibitor LCS-1 could significantly enhance the level of viral DNA replication and in-crease the mortality of CnmeGV-infected larvae.The above results indicated that the activation of FOXO and Cu/Zn SOD genes depended on the level of oxidative stress,and FOXO and Cu/Zn SOD genes played key roles in the antiviral defense of C.medinalis larvae.In addition,FOXO inhibitors also significantly attenuated the induction level of Cu/Zn SOD transcription by viral infection,and multiple FOXO binding sites were identified in the Cu/Zn SOD promoter region,indicating that FOXO could directly regulate the expression of Cu/Zn SOD.The results of this study provide a new method for the control of C.medinalis and the study of insect-virus interaction mechanism.关键词
稻纵卷叶螟/稻纵卷叶螟颗粒体病毒/氧化应激/FOXO转录因子/铜/锌超氧化物歧化酶Key words
Cnaphalocrocis medinalis/Cnaphalocrocis medinalis granulovirus/oxidative stress/transcription factor FOXO/copper/zinc superoxide dismutase(Cu/Zn SOD)分类
农业科技引用本文复制引用
张楠,韩光杰,刘琴,李传明,林曼曼,祁建杭,陆玉荣,夏杨,徐健..稻纵卷叶螟FOXO调控Cu/Zn SOD参与抗杆状病毒CnmeGV感染的机制[J].江苏农业学报,2025,41(12):2334-2340,7.基金项目
国家重点研发计划项目(2024YFD1400700) （2024YFD1400700）
国家自然科学基金项目(32302351、32372539) （32302351、32372539）
农业基础性长期性科技工作项目(NAES069AM04) （NAES069AM04）
江苏省农业科技自主创新基金项目[CX(24)1005] （24）
