兰州大学学报（医学版）2025，Vol.51Issue(11)：29-35,7.DOI:10.13885/j.issn.2097-681X.2025.11.005
康艾注射液影响结直肠癌奥沙利铂耐药的作用机制研究
Mechanism study of Kang'ai Injection on oxaliplatin chemoresistance in colorectal cancer
摘要
Abstract
Objective To investigate the mechanisms by which the Kang'ai Injection reverses oxaliplatin resis-tance in colorectal cancer using an integrated approach of network pharmacology,molecular docking and experi-mental validation.Methods Active ingredients of the Kang'ai Injection and their potential targets were retrieved from the TCMSP database,while oxaliplatin resistance-related targets in colorectal cancer were collected from GeneCards and other databases.The intersection targets between the two sets were identified.A "Kang'ai active ingredient-oxaliplatin resistance target" network was constructed to screen core active com-pounds,and a protein-protein interaction network was used to identify key targets.Gene Ontology and Kyoto Encyclope-dia of Genes and Genomes pathway enrichment analyses were performed using the DAVID data-base.Molecu-lar docking was carried out with AutoDock Vina to evaluate binding affinities between the key components and targets.In vitro,the human colon cancer cell line HCT116 and its oxaliplatin-resistant deriva-tive HCT116/L were cultured.Cell viability was assessed by CCK-8 assay,apoptosis was measured by flow cytometry and mRNA and protein expression levels of relevant genes determined by quantitative real-time PCR and Western blotting,respectively.Results A total of 184 overlapping targets between Kang'ai Injection and oxaliplatin-resistant colorectal cancer were identified.Core active ingredients included galangin and dihy-droisoflavone,and key targets encompassed TP53,AKT1 and EGFR.Gene Ontology enrichment analysis indi-cated that these targets were primarily involved in the regulation of apoptosis and cellular stress responses.Kyoto Encyclopedia of Genes and Genomes analysis revealed significant enrichment in the PI3K-AKT path-way.Molecular docking demonstrated strong binding affinities between the main active compounds and key target proteins.Experimental validation showed that Kang'ai Injection significantly inhibited the viability of HCT116/L cells,promoted apoptosis,upregulated TP53 mRNA expression,downregulated EGFR mRNA ex-pression and suppressed activation of the PI3K-AKT pathway in a dose-dependent manner.Conclusion Kang'ai Injection reverses oxaliplatin resistance in colorectal cancer in a dose-dependent manner,likely via upregulat-ing TP53,downregulating EGFR and inhibiting the PI3K-AKT pathway.关键词
康艾注射液/结直肠癌/奥沙利铂耐药/网络药理学/分子对接/PI3K-AKT[信号]通路Key words
Kangai Injection/colorectal cancer/oxaliplatin resistance/network pharmacology/molecular dock-ing/PI3K-AKT pathway分类
医药卫生引用本文复制引用
张馨,刘爱萍,刘明,徐彩花,张明悦,侯桃霞,徐明丽..康艾注射液影响结直肠癌奥沙利铂耐药的作用机制研究[J].兰州大学学报（医学版）,2025,51(11):29-35,7.基金项目
甘肃省高校教师创新基金项目(2025A-402) （2025A-402）
