畜牧与饲料科学2025，Vol.46Issue(6)：1-8,8.DOI:10.12160/j.issn.1672-5190.2025.06.001
基于反溶剂法的玉米醇溶蛋白-白藜芦醇纳米体系的制备、表征与抗氧化性能研究
Study on Preparation,Characterization,and Antioxidant Activity of Resveratrol-Zein Nanoparticle Systems Based on the Anti-Solvent Method
摘要
Abstract
[Objective]To study the preparation,characterization,and antioxidant activity of resveratrol-zein nanoparticle(Res-ZeinNPs)systems based on the anti-solvent method.[Methods]Res-ZeinNPs were prepared using the anti-solvent method,and the formulation was optimized via orthogonal design.The Res-ZeinNPs were systematically characterized,and their release profiles under different pH conditions and antioxidant activity were dynamically evaluated.[Results]Four factors-zein mass,ethanol volume,Res mass,and ultrapure water volume-showed highly significant effects on encapsulation efficiency(P<0.01).The optimal conditions for preparing Res-ZeinNPs were:zein mass 75 mg,70%ethanol 75 mL,Res mass 2.5 mg,and ultrapure water 200 mL.The resulting particles achieved an encapsulation efficiency of 98.13%and a drug loading capacity of 20.9%.Transmission electron microscopy observations revealed that Res-ZeinNPs were spherical,with a modal particle size of approximately 150 nm and a negative charge.In vitro release studies indicated that Res-ZeinNPs exhibited pH-responsive release characteristics;at pH 1.2 and 7.4,the 24 h cumulative release rates were 38.80%and 45.24%,respectively,both highly significantly lower(P<0.01)than raw Res powder.DPPH radical scavenging assays demonstrated that Res-ZeinNPs had a significantly higher(P<0.05)DPPH scavenging rate than raw Res powder.[Conclusion]Res-ZeinNPs exhibit high encapsulation efficiency,favorable sustained-release properties,and enhanced antioxidant activity,which can improve the stability and bioavailability of resveratrol.关键词
白藜芦醇/玉米醇溶蛋白/纳米颗粒/反溶剂法/正交设计/包封率Key words
resveratrol/zein/nanoparticles/anti-solvent method/orthogonal design/encapsulation efficiency分类
农业科技引用本文复制引用
闫婧怡,黄进,高梓博,蒋浩举,朱恒甲..基于反溶剂法的玉米醇溶蛋白-白藜芦醇纳米体系的制备、表征与抗氧化性能研究[J].畜牧与饲料科学,2025,46(6):1-8,8.基金项目
河南省自然科学基金项目(242300420142) （242300420142）
河南工业大学基金培育计划(2024PYJH014). （2024PYJH014）
