南京农业大学学报2026，Vol.49Issue(1)：73-83,11.DOI:10.7685/jnau.202409026
6种根际促生细菌对浙贝母生长与活性成分的影响
Effects of six rhizosphere growth-promoting bacterial strains on growth and active components of Fritillaria thunbergii
摘要
Abstract
[Objectives]The paper aimed to isolate the growth-promoting bacteria from the rhizosphere soil of Fritillaria thunbergii.The effects of 6 different rhizosphere growth-promoting bacteria on growth and active components of F.thunbergii were studied in order to provide basis for fertilizer and pesticide reduction application and ecological agriculture planting.[Methods]Various specific media were selected to screen the capacities of nitrogen fixation,phosphorus solubilization,potassium solubilization,IAA production and iron production of rhizosphere growth-promoting bacteria.Six growth-promoting bacteria(Serratia sp.TZ1,Bacillus sp.JJ2,Exiguobacterium sp.JJ15,Enterobacter sp.TZ21,Pantoea sp.TZ112 and Pseudomonas sp.JJ137)were applied in pot experiment,and the growth indexes,chlorophyll,osmotic regulatory substances and active components were determined to explore the growth promotion effect of rhizosphere growth-promoting bacteria on F.thunbergii.[Results]Six rhizosphere growth-promoting bacteria were screened from rhizosphere soil of F.thunbergii.Six growth-promoting bacteria had all significant effects on growth of F.thunbergii at different growth stages.Six rhizosphere bacteria could promote the growth of plant and increase the dry weight and fresh weight of leaves,stems,bulbs,etc.The leaf area of TZ1 group was 28.69%higher than CK group(no inoculation with soil rhizobacteria)at the beginning of depletion stage,and plant height of TZ112 group were 23.75%higher than CK group at the beginning of depletion stage.Rhizosphere growth-promoting bacteria also had significant effects on the contents of chlorophyll and osmoregulatory substances in the leaves of F.thunbergii.The contents of chlorophyll a,chlorophyll b and total chlorophyll in TZ1 group at the beginning of depletion stage increased most significantly,which were 14.70%,15.69%and 14.51%higher than those in CK group,respectively.Six rhizosphere bacteria were beneficial to the accumulation of active components(peimine and peiminine)of F.thunbergii during the dormant period.Among them,bulb biomass per plant,peiminine content and total active component biomass per plant in TZ112 group increased most significantly,which were 18.83%,30.12%and 33.01%higher than those in CK group,respectively.[Conclusions]There were growth-promoting bacteria with a variety of characteristics in the rhizosphere of F.thunbergii.The isolated and screened bacteria TZ1,JJ2,JJ15,TZ21,TZ112 and JJ137 had the ability to promote the growth and physiological process of F.thunbergii,among which TZ112 had the best comprehensive capability.关键词
浙贝母/根际促生菌/促生能力/生长/活性成分Key words
Fritillaria thunbergii/rhizosphere growth promoting bacteria/growth promoting ability/growth/active components分类
农业科技引用本文复制引用
高阳,孙莉琼,李晓帆,唐晓清,王康才..6种根际促生细菌对浙贝母生长与活性成分的影响[J].南京农业大学学报,2026,49(1):73-83,11.基金项目
国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
横向合作-中药材种植技术与基地建设指导项目(20231020) （20231020）
