南京农业大学学报2026，Vol.49Issue(1)：94-102,9.DOI:10.7685/jnau.202411023
硝化抑制剂DMPP对滨海盐碱水稻土细菌群落的影响
Effects of the nitrification inhibitor DMPP on bacterial communities in coastal saline-alkali paddy soils
摘要
Abstract
[Objectives]This study aimed to investigate the effects of nitrification inhibitor(3,4-dimethylpyrazole phosphate,DMPP)on bacterial community diversity,structure,and assembly mechanisms in coastal saline-alkaline paddy soils.[Methods]A pot experiment combined with 16S rRNA gene amplicon high-throughput sequencing was conducted to analyze the impacts of DMPP application on bacterial community structure and assembly processes in soils with two salinity levels.[Results]Compared with low-salinity soil,high-salinity soil exhibited lower Shannon index and reduced community stability.DMPP application increased Shannon and Chao1 indices while enhancing community stability in high-salinity soil,whereas it decreased these indices and stability in low-salinity soil.Significant structural differences were observed between soil treatments.High-salinity soil showed lower relative abundances of Myxococcota and Acidobacteriota but higher abundances of Proteobacteria and Bacteroidota compared to low-salinity soil.DMPP addition significantly reduced Bacteroidota and Chloroflexi abundances while increasing Actinobacteria and Proteobacteria abundances.Stochastic processes predominantly governed bacterial community assembly.DMPP amplified the relative contribution of stochasticity in high-salinity conditions but reduced it under low-salinity conditions.Redundancy analysis and correlation analysis revealed that soil electrical conductivity(P<0.001),organic matter(P<0.01),and available nitrogen(P<0.001)were key environmental factors influencing bacterial community structure.[Conclusions]DMPP application significantly altered bacterial communities in coastal saline-alkaline paddy soils with varying salinity levels.This study provides theoretical foundation for enhancing nitrogen utilization efficiency in coastal saline-alkaline ecosystems.关键词
高通量测序/细菌群落多样性/群落组装机制/3,4-二甲基吡唑磷酸盐/滨海/盐碱地Key words
high-throughput sequencing/bacterial community diversity/community assembly mechanisms/3,4-dimethylpyrazole phosphate(DMPP)/coastal/saline-alkali land分类
农业科技引用本文复制引用
龙翔,刘浩,朱诗君,姚红燕,曹慧,汪峰..硝化抑制剂DMPP对滨海盐碱水稻土细菌群落的影响[J].南京农业大学学报,2026,49(1):94-102,9.基金项目
国家自然科学基金项目(32471725) （32471725）
宁波市"科技创新2025"重大专项(2022Z169,2023Z114) （2022Z169,2023Z114）
