水产学报2026，Vol.50Issue(3)：126-137,12.DOI:10.11964/jfc.20240714601
海洋细菌胞外多糖可拉酸提取及其对厚壳贻贝稚贝附着的作用
Extraction of colanic acid of marine bacteria and its effects on the settlement of Mytilus coruscus plantigrades
摘要
Abstract
To investigate the mechanism by which the exopolysaccharide colanic acid(CA)from marine bacteria induces the attachment of Mytilus coruscus,juvenile M.coruscus were used as experimental subjects.The optimal culture conditions for CA production were explored,followed by extraction,purification,and monosaccharide composition analysis of CA.Different concentrations of CA were applied to induce mussel settlement,and quantitative real-time polymerase chain reaction(qRT-PCR)was performed after CA injection to evaluate the byssus attachment efficiency.In this study,a genetically engineered strain of Pseudoalteromonas marina ECSMB14103 Δ01912 with high CA production was used.Through single-factor experi-ments and Box-Benhnken central composite design optimization of extraction culture conditions,the optimal cultivation scheme for CA production in this strain was determined.The optimal scheme for CA production is a pH of 6.0,cultivation tem-perature of 30℃,cultivation time of 18 h,and inoculum of 8%.Ion chromatography analysis revealed that the product con-sisted of a heteropolysaccharide composed of fucosylglucose,mannose,glucose,and glucuronic acid in a molar ratio of 2∶2∶1∶1.CA concentrations of 20 μg/mL and 50 μg/mL embedded on glass slides,resulted in the highest inducing activity of M.coruscus plantigrades.Injection of purified CA into mussels showed significant up-regulation of the expression levels of Mcfp-1P,Mcfp-2 and Mcfp-5 genes,which encode adhesive proteins in byssal threads.No significant changes were observed in the expression levels of the Mcfp-1T gene after treatment with CA.Furthermore,the expression level of the Mccol2 gene signi-ficantly increased,while Mccol3 expression significantly decreased.These findings suggest that CA may regulate the settle-ment of M.coruscus plantigrades through promoting secretion of adhesive proteins from byssal threads,and modulating differ-ent types of collagen proteins.This study provides a theoretical basis for exploring the mechanism of inducing the settlement of M.coruscus and has potential value in applying CA-induced settlement to the aquaculture of M.coruscus.关键词
厚壳贻贝/可拉酸/提取优化/附着/足丝蛋白Key words
Mytilus coruscus/colanic acid/extraction optimization/settlement/byssus proteins分类
农业科技引用本文复制引用
吴叶凡,解静仪,彭莉华,杨金龙,梁箫..海洋细菌胞外多糖可拉酸提取及其对厚壳贻贝稚贝附着的作用[J].水产学报,2026,50(3):126-137,12.基金项目
国家重点研发计划(2023YF2401902,2023YFE0115500) （2023YF2401902,2023YFE0115500）
国家自然科学基金(4186159) National Key Research and Development Program of China(2023YFD2401902,2023YFE0115500) （4186159）
National Natural Science Foundation of China(41876159) （41876159）
