热带农业工程2025，Vol.49Issue(6)：30-34,5.
二糖对农杆菌室温保存后存活率的影响
Effect of Disaccharides on the Survival Rate of Agrobacterium after Room Temperature Storage
摘要
Abstract
To explore the preservation efficacy of disaccharide compounds on Agrobacterium under room temperature conditions.Agrobacterium cells were placed in protectants with different types and concentrations of disaccharide and stored at room temperature to determine their survival rate and ability to infect plants.Agrobacterium survival rate survival rate remains unchanged when stored in a protective agent with a sucrose content of 20%～25%in storage period of 45 days.Survival rate survival rate is higher when the 20%～25%concentration of trehalose used in the protective with a storage period of 45 days for Agrobacterium.In the protective agent containing 20%trehalose and 10%protein,Agrobacterium has a higher survival rate that can be maintained for 45 days,while the transient positive rate of GUS gene expression can still be used for the ability to infect plants.These findings provide insights for enhancing gene high-throughput transformation efficiency and offer valuable references for developing commercial microbial products suitable for ambient-temperature storage.关键词
农杆菌/保护剂/海藻糖/蔗糖/贮存Key words
Agrobacterium/protectant agent/trehalose/sucrose/storage分类
农业科技引用本文复制引用
刘晓星,杨芳丽,黄惠娜,杨雨婷,陈怡洁,王晞..二糖对农杆菌室温保存后存活率的影响[J].热带农业工程,2025,49(6):30-34,5.基金项目
广西农科院稳定资助科研团队项目(No.桂科农2021YT095). （No.桂科农2021YT095）
