水产学报2026，Vol.50Issue(3)：138-152,15.DOI:10.11964/jfc.20250715070
高脂饲料中添加丙酸钠对大口黑鲈幼鱼生长、肝脏健康及肠道菌群的影响
Effects of sodium propionate supplementation on growth,liver health,and intestinal flora of juvenile largemouth bass(Micropterus salmoides)in a high-fat diet
摘要
Abstract
Fat is a necessary nutrient for the growth of fish,but diet with a high fat level can lead to decreased growth perform-ance,metabolic disorders,and fat accumulation.Propionic acid is one of the most abundant short-chain fatty acids in the intest-ine and has the potential to promote growth,enhance immunity,and regulate lipid metabolism.Sodium propionate,as its diet additive form,has received little research on its role in mitigating the negative effects of high-fat diets on fish health.To invest-igate the effects of sodium propionate on the growth,liver healthy and intestinal flora of largemouth bass(Micropterus sal-moides)in a high-fat diet,a total of 270 juvenile M.salmoides with an initial body weight of(2.69±0.04)g were selected and randomly divided into 3 groups,with 3 replicates in each group.They were respectively fed with 3 types of isonitrogenous diets:control diet(CON group,10%fat),high-fat diet(HFD group,18%fat),and high-fat diet supplemented with sodium pro-pionate(HFD+NaSP group,18%fat+5 g/kg sodium propionate)for 56 days.The results showed that compared with the HFD group,the HFD+NaSP group exhibited significantly increased weight gain rate and specific growth rate.Serum triglyceride,total cholesterol content,as well as the activities of alanine aminotransferase and aspartate aminotransferase were significantly reduced in the HFD+NaSP group compared to the HFD group.The HFD+NaSP group demonstrated significantly elevated total antioxidant capacity,catalase,and superoxide dismutase activities,while malondialdehyde content was significantly reduced compared to the HFD group.Compared with the HFD group,the activities of intestinal lipase,amylase and pepsin in the HFD+NaSP group were significantly increased,the HFD+NaSP group showed significant upregulation of pparα and cpt1 expression in the liver,along with significant downregulation of srebp1,fas,acc tnf-α,il-1β,il-8 caspase3,caspase9,and bax expression.Regarding intestinal flora,the HFD+NaSP group displayed significantly higher Shannon and Simpson indices com-pared to the HFD group,while Ace and Chao indices showed no significant differences,the HFD+NaSP group significantly reduced the relative abundance of Mycoplasma while increasing Leifsonia abundance.In conclusion,dietary NaSP supplement-ation ameliorated the adverse effects of high-fat diet on the growth performance,liver health,and intestinal flora of M.sal-moides.To provide theoretical references for promoting the application practice of sodium propionate in high-fat feed for fish.关键词
大口黑鲈/高脂/丙酸钠/生长/肠道菌群Key words
Micropterus salmoides/high-fat/sodium propionate/growth/intestinal flora分类
农业科技引用本文复制引用
陈林辉,唐延杰,严盈,陈萱,冯德祥,黎明..高脂饲料中添加丙酸钠对大口黑鲈幼鱼生长、肝脏健康及肠道菌群的影响[J].水产学报,2026,50(3):138-152,15.基金项目
国家自然科学基金(32473130) （32473130）
广西水产生物技术与现代生态养殖重点实验室开放基金(23-026-1) （23-026-1）
河南省现代农业产业技术体系建设专项(HARS-22-16-G3) National Natural Science Foundation of China(32473130) （HARS-22-16-G3）
Open Fund of Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture(23-026-1) （23-026-1）
Special Fund for the Construction of Modern Agricul-tural Industry Technology System in Henan Province(HARS-22-16-G3) （HARS-22-16-G3）
