水产学报2026，Vol.50Issue(3)：171-183,13.DOI:10.11964/jfc.20240414492
饲料中添加不同水平纳米氧化铁对黄颡鱼幼鱼肌肉发育的影响及其机理
Effects and mechanism of different levels of Fe2O3 nanoparticles on muscle development in juvenile yellow catfish(Pelteobagrus fulvidraco)
摘要
Abstract
To investigate the effects of different levels of nano-iron oxide on the muscle development of young yellow catfish(Pelteobagrus fulvidraco),using Fe2O3 NPs as the Fe sources,the present study formulated three experimental diets with diet-ary Fe levels at 22.23(control),44.53(Low Fe2O3 NPs group),61.85 mg Fe/kg diet(High Fe2O3 NPs group),respectively,and fed to P.fulvidraco for 10 weeks.Our aims were to investigate whether and how different levels of Fe2O3 NPs affected the muscle development of juvenile P.fulvidraco.Low dietary Fe2O3 NPs level significantly augmented muscle fiber diameter,stimulated protein deposition,decreased NEFA and MDA levels in muscle tissue,elevated GSH content,upregulated the mRNA expression of MRF family members and fsp1 and slc7a11 genes,downregulated the mRNA expression of mstn gene and inhibited both mRNA and protein expressions of inflammatory factor nf-κb1 gene.The high Fe2O3 NPs diets inhibited muscle fiber diameter,increased the contents of iron,NEFA and MDA,induced GSH depletion,stimulated the expression of ferroptosis related genes and inhibited the expression of fsp1 and slc7a11 genes,and increased mRNA and protein expression of inflammatory factor nf-κb1 gene.Moreover,we cloned the sequences of myog and slc7a11 promoters from yellow catfish,and predicted the potential binding sites for NF-κB1 transcription factor on these two promoters.The dual luciferase activity assay and EMSA indicated that the NF-κB1 transcription factor directly binds to the myog promoter(-6 bp/-16 bp)and the slc7a11 promoter(-430 bp/-439 bp).Furthermore,NF-κB1 transcription factor plays a direct role in mediating the transcription and reg-ulation of these promoters;Fe2O3 NPs incubation enhanced this binding.This study revealed the potential mechanism of the regulation of muscle development by Fe2O3 NPs,and provided a good basis for dietary Fe2O3 NPs addition in the aquafeeds.关键词
黄颡鱼/纳米氧化铁/肌肉发育/转录调控Key words
Pelteobagrus fulvidraco/Fe2O3 NPs/muscle development/transcription and regulation分类
农业科技引用本文复制引用
张天华,魏晓雷,郭嘉程,刘璐璐,段芙萱,罗智..饲料中添加不同水平纳米氧化铁对黄颡鱼幼鱼肌肉发育的影响及其机理[J].水产学报,2026,50(3):171-183,13.基金项目
国家自然科学基金(32220103013) （32220103013）
国家重点研发计划(2023YFD2400600) National Natural Science Foundation of China(32220103013) （2023YFD2400600）
National Key R&D Program of China(2023YFD2400600) （2023YFD2400600）
