北方农业学报2025，Vol.53Issue(6)：55-65,11.DOI:10.12190/j.issn.2096-1197.2025.06.05
多功能秸秆降解菌LF-4的鉴定及功能验证
Identification and functional verification of the multifunctional straw-degrading strain LF-4
摘要
Abstract
[Objective]To identify and verify the functions of the multifunctional straw-degrading strain LF-4,addressing issues such as slow straw decomposition,excessive residue,and potential threats to subsequent crops during the process of straw returning to the field.[Methods]Strain LF-4 was isolated from rapeseed straw.Its cellulose-degrading capability was measured using the Congo Red staining method,aniline blue decolorization method,and filter paper disintegration assay.The strain was identified through morphological and molecular biological methods.Its cellulase activity and straw degradation capability were determined.The growth-promoting,antagonistic,and phosphate-solubilizing functions of the strain were verified using the salkowski colorimetric method and the plate confrontation assay.[Results]Strain LF-4 exhibited robust cellulose-degrading capacity and was identified as Bacillus velezensis.Its peak cellulase activity reached 207.45 U/mL,and the indoor degradation rate of rapeseed straw reached 53.04%after 30 d of liquid fermentation.Strain LF-4 demonstrated growth-promoting,phosphate-solubilizing,and protease-producing functions,with an indole-3-acetic acid(IAA)yield of 10.26 mg/L,and displayed a broad antimicrobial spectrum,with a maximum inhibition rate of 67.19%.[Conclusion]The core characteristic of B.velezensis LF-4 is the strong cellulose-degrading capability,and it also possesses growth-promoting,antagonistic,and phosphate-solubilizing functions.关键词
秸秆/降解菌/纤维素降解/促生/贝莱斯芽孢杆菌Key words
Straw/Degrading strain/Cellulose degradation/Growth-promotion/Bacillus velezensis分类
农业科技引用本文复制引用
余江泳,王东,侯伟峰,郭晨,贺小勇,张超,赵颖,赵远征..多功能秸秆降解菌LF-4的鉴定及功能验证[J].北方农业学报,2025,53(6):55-65,11.基金项目
内蒙古自治区直属高校基本科研业务费(BR22-11-14) （BR22-11-14）
内蒙古中央引导地方发展基金项目(2024ZY0028) （2024ZY0028）
呼和浩特市高校院所协同创新项目(XTCX2023-19) （XTCX2023-19）
