北方农业学报2025，Vol.53Issue(6)：77-85,9.DOI:10.12190/j.issn.2096-1197.2025.06.07
枯草芽孢杆菌和生物炭对盐碱土改良及月季生长的影响
Effects of Bacillus subtilis and biochar on saline-alkali soil improvement and the growth of Rosa chinensis
摘要
Abstract
[Objective]To explore the effects of the combined application of Bacillus subtilis and biochar on the improvement of saline-alkali soil and the growth of Rosa chinensis in Qingdao,Shandong Province.[Methods]A pot experiment of R.chinensis was conducted using saline-alkali soil collected from the Dongjiakou Port area in Qingdao.Four soil treatments were established:application of B.subtilis suspension(Bs),2%biochar(Bi),B.subtilis suspension+2%biochar(Bs+Bi),and untreated soil as the control(CK).The effects of different treatments on soil physicochemical properties,enzyme activities,and the growth and physiological characteristics of R.chinensis were analyzed.[Results]Compared with CK,the Bs+Bi treatment significantly reduced soil pH value and electrical conductivity by 4.80%and 14.01%,respectively(P<0.05),while significantly increasing the contents of soil nitrate nitrogen,available phosphorus,and organic matter by 83.28%,11.68%,and 65.13%,respectively(P<0.05);soil nitrate reductase and nitrite reductase activities were significantly increased by 7.53%and 21.38%,respectively(P<0.05).Compared with CK,the Bs treatment significantly increased plant height and belowground biomass of R.chinensis by 17.93%and 20.10%,respectively(P<0.05),and the Bi treatment significantly increased aboveground biomass by 29.74%(P<0.05);under the Bs+Bi treatment,chlorophyll a and carotenoid contents significantly increased by 23.29%and 79.19%(P<0.05),and soluble sugar and soluble protein contents increased by 13.02%and 34.07%,respectively.[Conclusion]The combined application of B.subtilis and biochar can effectively improve the physicochemical properties of saline-alkali soil,promote soil nitrogen cycling,and enhance soil enzyme activities.Although this combined application has no obvious effect on the R.chinensis growth,it can significantly increase the photosynthetic pigment contents and accumulate osmotic adjustment substances.关键词
枯草芽孢杆菌/生物炭/盐碱土改良/月季/生长/生理特性Key words
Bacillus subtilis/Biochar/Saline-alkali soil improvement/Rosa chinensis/Growth/Physiological characteristics分类
农业科技引用本文复制引用
王正喜,白晶,王汝法,宋京晖,尹清苓,隋龙,陈锐,张迪..枯草芽孢杆菌和生物炭对盐碱土改良及月季生长的影响[J].北方农业学报,2025,53(6):77-85,9.基金项目
国家自然科学基金项目(32471942) （32471942）
国家自然科学基金青年科学基金项目(32101582) （32101582）
基础研究计划(自然科学基金)——青年基金项目(BK20210613) （自然科学基金）
