四川医学2025，Vol.46Issue(12)：1327-1333,7.DOI:10.16252/j.cnki.issn1004-0501-2025.12.002
LINC00461调节miR-485-3p/NRP1轴对黑色素瘤细胞增殖、迁移和侵袭的影响
Effects of LINC00461 on the Proliferation,Migration,and Invasion of Melanoma Cells via Regulating the miR-485-3p/NRP1 Axis
摘要
Abstract
Objective To investigate the effects of long non-coding RNA00461(LINC00461)on the proliferation,migra-tion,and invasion of melanoma cells by regulating microRNA-485-3p(miR-485-3p)/neuronilin-1(NRP1).Methods qRT-PCR was used to detect the expression of LINC00461,miR-485-3p,and NRP1 mRNA in cutaneous melanoma(CM)tissue and normal skin tissue excised during surgery in our hospital from September 2022 to December 2023.A2058 cells were studied and divided into the following groups:Control,sh-NC,sh-LINC00461,sh-LINC00461+anti-NC,and sh-LINC00461+anti-miR-485-3p.The ex-pression of LINC00461,miR-485-3p,and NRP1 mRNA was detected in each group.CCK8 and EdU staining were applied to detect the proliferation of A2058 cells.Scratch experiment was applied to detect the migration of A2058 cells.Transwell experiment was applied to detect the invasion of A2058 cells.Western blot was applied to detect the expression of PCNA,NRP1,and MMP-2 pro-teins in A2058 cells.Dual luciferase reporter gene assay was applied to detect the interaction between LINC00461 and miR-485-3p,and between miR-485-3p and NRP1.Results The expression of LINC00461 and NRP1 mRNA in CM tissue was higher than that in adjacent cancer tissue,and the expression of miR-485-3p was lower than that in adjacent cancer tissue(P<0.05).The OD450 value,EdU positive cell rate,scratch healing rate,invasion number,LINC00461,NRP1 mRNA and protein,PCNA pro-tein,MMP-2 protein expression of A2058 cells in sh-LINC00461 group were lower than those in sh-NC group and Control group,the expression of miR-485-3p was higher in the sh-NC group and the Control group(P<0.05).Compared with the sh-LINC00461 group and the sh-LINC00461+anti-NC group,the OD450 value,EdU positive cell rate,scratch healing rate,invasion number,NRP1 mRNA and protein,PCNA protein,MMP-2 protein expression in the sh-LINC00461+anti-miR-485-3p group were higher,the ex-pression of miR-485-3p was lower(P<0.05).LINC00461 was able to negatively regulate miR-485-3p,while miR-485-3p was a-ble to negatively regulate NRP1.Conclusion Knockdown of LINC00461 can inhibit the proliferation,migration,and invasion of melanoma cells,and this mechanism may be achieved by regulating the miR-485-3p/NRP1 signaling axis.关键词
长链非编码RNA00461/微小RNA-485-3p/神经纤毛蛋白1/黑色素瘤/增殖/迁移/侵袭Key words
long chain non coding RNA00461/micro RNA-485-3p/neuronilin-1/melanoma/proliferation/migration/inva-sion分类
医药卫生引用本文复制引用
吴一文,胡友红,周文文,陈圆圆,任芬芬,方木平..LINC00461调节miR-485-3p/NRP1轴对黑色素瘤细胞增殖、迁移和侵袭的影响[J].四川医学,2025,46(12):1327-1333,7.基金项目
湖北省卫健委基金课题(编号:WJ2017M238) （编号:WJ2017M238）
