山东农业科学2025，Vol.57Issue(12)：23-30,8.DOI:10.14083/j.issn.1001-4942.2025.12.004
苹果'烟富6号'与'红丹'杂交F1代红肉性状的早期筛选研究
Early Selection of Red-Fleshed Trait in F1 Hybrid Population of Apple'Yanfu 6'×'Hongdan'
摘要
Abstract
The breeding cycle of apple is long,and early selection of hybrid offspring is an important procedure in apple genetic improvement and new variety breeding.'Hongdan'is a new red-fleshed apple vari-ety with red skin cultivated by our research group(Apple Breeding Group,Qingdao Agricultural University)through artificial hybridization.In order to improve the small fruit size of'Hongdan',our research group used the late-maturing red-skin and white-fleshed variety'Yanfu 6'(male parent)to cross with red-skin red-fleshed'Hongdan'(female parent)to obtain the F1 hybrid population.In this study,the genotypes of Md-MYB1/10 of the two parents and F1 hybrids were analyzed,and the anthocyanin content in leaves and the ex-pression of related genes in the anthocyanin synthesis pathway were determined.The results showed that Md-MYB1/10 had the highest expression in the parent'Hongdan'and the F1 population with red leaves and dark-red stems.Three types of seedlings in F1 hybrid were identified,namely,green leaves and green stems,green leaves and light-red stems,and red leaves and dark-red stems.It was found that only the individuals with red leaves and dark-red stems had both R1R6:MYB10 and Red-TE insertion.Therefore,we could select individu-als with red leaves and dark-red stems at seedling stage,which would be used for breeding new varieties with red skin and red flesh from these individuals.This study could provide a theoretical foundation for early selec-tion of red-fleshed apple lines,and improve the efficiency of cross breeding.关键词
红肉苹果/杂交F1代/MdMYB1/10/早期筛选/R1R6Key words
Red-fleshed apple/F1 Hybrids/MdMYB1/10/Early selection/R1R6分类
农业科技引用本文复制引用
刘梦媛,徐吉花,刘思瀚,赵水清,徐以诺,张虎,王菲,吕娟,徐金铭,张玉刚..苹果'烟富6号'与'红丹'杂交F1代红肉性状的早期筛选研究[J].山东农业科学,2025,57(12):23-30,8.基金项目
山东省自然科学基金项目(ZR2023MC169) （ZR2023MC169）
山东省农业良种工程项目(2023LZGCQY007) （2023LZGCQY007）
山东省泰山学者人才工程建设项目(tstp20221134) （tstp20221134）
国家现代苹果产业技术体系项目(CARS-27) （CARS-27）
山东省大学生创新创业训练项目(S202510435041) （S202510435041）
