山东农业科学2025，Vol.57Issue(12)：41-47,7.DOI:10.14083/j.issn.1001-4942.2025.12.006
移栽期和打顶时期对烤烟上部叶碳代谢及相关基因表达量的影响
Effects of Transplanting Time and Topping Period on Carbon Metabolism and Related Gene Expression in Upper Leaves of Flue-Cured Tobacco
摘要
Abstract
To explore the optimal transplanting time and topping period combination for Yunyan 87 flue-cured tobacco in the Heishi tobacco area of Weining,and to further improve the quality of upper tobacco leav-es,this experiment used a two-factor randomized block design with transplanting time and topping period as factors.Three transplanting times(April 10,April 20,April 30)and three topping periods(budding,early flowering,full flowering)were set,forming a total of nine combination treatments(T1 to T9),and their inter-action effects on content of carbon metabolites,key enzyme activities,and related gene expression in upper leaves of flue-cured tobacco were studied.The results showed that under different transplanting times,topping at early flowering was the most favorable period for promoting the accumulation of carbon metabolites(total sugar,reducing sugar and starch)in upper leaves with the treatment of topping in early flowering period and transplanting on April 30(T8)showing the best.The activity of invertase(INV)in upper leaves was higher in the treatments of topping in budding period and transplanting on April 10(T1)and topping in early flower-ing period and transplanting on April 20(T5).The sucrose synthase(SS)activity was higher in the treat-ments of T7(topping in budding period and transplanting on April 30)and T8.The relative expression of the INV gene NtINV was higher in T5 and T9(topping in full flowering period and transplanting on April 30)treatments.The relative expression of the SS gene NtSS was higher in the treatments of T6(topping in full flowering period and transplanting on April 20)and T9.The contents of total sugar,reducing sugar and starch in cured upper leaves were higher in T3(topping in full flowering period and transplanting on April 10)and T8 treatments,and the chemical composition coordination performed better,which were beneficial for impro-ving tobacco leaf quality.The results of correlation analysis showed that the total sugar content in fresh tobacco leaves was significantly positively correlated with the starch content of cured tobacco leaves,and the reducing sugar content of fresh tobacco leaves was extremely significantly positively correlated with the starch content of cured tobacco leaves.In summary,it could be concluded that transplanting on April 30 combined with topping in early flowering periodwas the most beneficial for the carbon metabolism activity and quality improvement of upper tobacco leaves in the Heishi tobacco area of Weining.关键词
烤烟/移栽期/打顶时期/碳代谢/关键酶活性/基因表达Key words
Flue-cured tobacco/Transplanting time/Topping period/Carbon metabolism/Key enzyme activity/Gene expression分类
农业科技引用本文复制引用
张宇,钱雪松,吴学巧,李志能,郜军艺,李鹏志,熊晶,高焕晔..移栽期和打顶时期对烤烟上部叶碳代谢及相关基因表达量的影响[J].山东农业科学,2025,57(12):41-47,7.基金项目
贵州省烟草公司毕节市公司项目(2023520500240160) （2023520500240160）
贵州省烟草提质增效全省重点实验室建设项目(黔科合平台ZSYS[2025]028) （黔科合平台ZSYS[2025]028）
