山东农业科学2025，Vol.57Issue(12)：48-53,6.DOI:10.14083/j.issn.1001-4942.2025.12.007
萘乙酸喷施时期与浓度对烤烟上部叶碳代谢产物含量及其关键酶活性的影响
Effects of Spraying Period and Concentration of Naphthylacetic Acid on Carbon Metabolite Contents and Key Enzyme Activities in Upper Leaves of Flue-Cured Tobacco
摘要
Abstract
In order to explore the metabolic mechanism of spraying exogenous naphthylacetic acid(NAA)on the formation of availability in the upper leaves of flue-cured tobacco,Yunyan 87 was used as test material in this study.A two-factor randomized block trial with 12 treatments of NAA spraying period(on the topping day,at 15 days after topping,at 30 days after topping)and spraying concentration(0,10,30,50 mg/L)was designed,and their effects on content of carbon metabolites,key enzymes activities and related gene expressions in the upper leaves of flue-cured tobacco were studied.The results showed that spraying high concentration of NAA on the topping day and spraying low concentration of NAA at 15 days after topping could promote the accumulation of starch in the upper leaves,and spraying NAA at 15 days after topping could pro-mote the accumulation of total sugar and reducing sugar,while spraying NAA at 30 days after topping reduced the content of total sugar and reducing sugar.In terms of key enzymes activities,the sucrose synthetase(SS)and sucrose phosphate synthase(SPS)activities were the highest when NAA was sprayed with medium high concentration at 15 days after topping,but the invertase(INV)activity of the upper leaves were inhibited by spraying NAA.In terms of the expression levels of key enzyme genes,spraying NAA reduced the expression levels of SS gene NtSS and INV gene NtINV.In conclusion,under the ecological conditions in Weining tobacco area,Bijie City,Guizhou Province,spraying 30 mg/L NAA at 15 days after topping was the most beneficial to improve the key enzymes activities of carbon metabolism in the upper leaves of Yunyan 87,increas the expres-sion of enzyme genes,and coordinate the carbon metabolism process during the maturity of upper leaves of to-bacco.关键词
烤烟/萘乙酸/上部叶/碳代谢Key words
Flue-cured tobacco/Naphthylacetic acid(NAA)/Upper leaves/Carbon metabolism分类
农业科技引用本文复制引用
龙尚沅,黄人杰,张宇,熊晶,吴学巧,郜军艺,李鹏志,高焕晔..萘乙酸喷施时期与浓度对烤烟上部叶碳代谢产物含量及其关键酶活性的影响[J].山东农业科学,2025,57(12):48-53,6.基金项目
贵州省烟草公司毕节市公司项目(2023520500240160) （2023520500240160）
贵州省烟草提质增效全省重点实验室建设项目(黔科合平台ZSYS[2025]028) （黔科合平台ZSYS[2025]028）
