山西中医药大学学报2025，Vol.26Issue(12)：1314-1321,8.DOI:10.19763/j.cnki.2096-7403.2025.12.03
白术七物颗粒调控肠道菌群代谢产物短链脂肪酸及结肠组织c-Kit表达治疗慢传输型便秘的机制研究
Mechanism of Baizhu Qiwu Granules in treating slow transit constipation by regulating intestinal flora metabolite SCFAs and c-Kit expression
摘要
Abstract
Objective:To investigate the effect of Baizhu Qiwu Granules on the expression of short-chain fatty acids(SCFAs)and c-Kit in colon tissue of model mice with slow transit constipation(STC),and to explore the relationship be-tween the target of Baizhu Qiwu Granules and SCFAs of intestinal flora.Methods:Forty-two specific pathogen-free(SPF)ICR mice were used in the experiment.They were randomly divided into a control group(n=9)and a modeling group(n=33)using a random number table.An STC mouse model was established by intragastric administration of loperamide hydrochlo-ride for 7 days.After modeling,three mice were randomly selected from each group for model validation.Upon successful validation,the remaining mice(control group:n=6;modeling group:n=30)were used.Mice in the modeling group were fur-ther randomly divided into five groups(n=6 each):the model group,low-dose Baizhu Qiwu Granules group,medium-dose Baizhu Qiwu Granules group,high-dose Baizhu Qiwu Granules group and prucalopride group.The mice in each group were given the corresponding drugs by gavage for 14 days.General signs were observed.Fecal water content and intestinal propul-sion rate were measured.Colon tissue morphology was examined using HE staining,c-Kit expression in colon tissue was de-tected by immunohistochemistry,and SCFAs content in mouse feces was analyzed by gas chromatography-mass spectrometry.Results:Compared with the control group,mice in the model group showed decreased activity,dull and rough fur,slow reac-tion,mental fatigue,reduced fecal output with hard,beaded or pellet-like stools,significantly decreased fecal water content,and a markedly lower intestinal propulsion rate,with the differences being statistically significant(P<0.001).Goblet cells in the colon mucosa were reduced,and c-Kit expression was significantly decreased,with the differences being statistically significant(P<0.01).Compared with the model group,mice in all Baizhu Qiwu Granules dose groups and the prucalopride group showed improved activity and mental state,restored fur gloss,quicker reactions,increased fecal output with soft,rugby ball-shaped stools,significantly increased fecal water content and intestinal propulsion rate,with the differences being statisti-cally significant(P<0.05,P<0.01).The number of goblet cells in colon tissue was restored,c-Kit expression was signifi-cantly increased,and the levels of butyric,hexanoic and valeric acids in feces were elevated,with the differences being sta-tistically significant(P<0.01).Conclusion:Baizhu Qiwu Granules can alleviate symptoms and promote intestinal motility in STC mice.The mechanism may be associated with upregulating c-Kit expression and regulating the intestinal flora metabo-lite SCFAs.关键词
白术七物颗粒/慢传输型便秘/c-Kit/短链脂肪酸Key words
Baizhu Qiwu Granules/STC/c-Kit/SCFAs分类
医药卫生引用本文复制引用
林仁敬,张佩雯,陈延杰,鲁海燕,罗敏..白术七物颗粒调控肠道菌群代谢产物短链脂肪酸及结肠组织c-Kit表达治疗慢传输型便秘的机制研究[J].山西中医药大学学报,2025,26(12):1314-1321,8.基金项目
湖南省自然科学基金面上项目(2024JJ5312) （2024JJ5312）
湖南省卫生健康委科研计划项目(B202303056408) （B202303056408）
湖南中医药大学研究生创新项目(202CX126) （202CX126）
