现代农业研究2025，Vol.31Issue(10)：60-69,10.
钾镁营养对茶树EGCG生物合成调控作用研究
Study on the Regulatory Effects of Potassium and Magnesium Nutrition on EGCG Biosynthesis in Tea Plants
摘要
Abstract
Epigallocatechin gallate(EGCG)is a key bioactive and quality-determining component in tea leaves,while the potassium and magnesium elements abundant in tea leaves are closely related to quality.They are integral parts of various enzyme proteins in the tea plant and important active substances in enzymatic reactions.However,there have been no systematic studies investigating the effects of potassium and magnesium nutrition on EGCG biosynthesis.This study used one-year-old tea seedlings of'Huangdan'as the test material.The experiment was conducted from January 2023 to October 2024.Using 0.15 mmol·L-1 MgSO4 and 1.0 mmol·L-1 K2SO4 as median values,a response surface methodology was employed to design a CCD experiment,treating the tea seedlings with different combinations of potas-sium and magnesium concentrations.The results showed that the KMg6 group(1 mmol/L K+and 0.15 mmol/L Mg)was the optimal potassium-magnesium ratio for EGCG synthesis.At the metabolomic level,the KMg6 group(medium potas-sium and magnesium)significantly increased the precursors of EGCG synthesis.In contrast,the KMg7 group(potassium only)resulted in lower precursor levels compared to the KMg6 group and increased the EGCG degradation product,gallic acid.Meanwhile,downstream metabolites of the flavonoid pathway were significantly upregulated under the KMg2(low potassium and magnesium)treatment.At the transcriptomic level,the expression levels of key genes involved in EGCG synthesis(CsPAL,CsCHS,CsF3H,CsDFR)were upregulated to varying degrees under different concentration treatments,and transcription factors from the MYB and bHLH families were also upregulated.However,the KMg4 group(high potassium and magnesium)significantly suppressed the expression of the aforementioned genes.关键词
钾镁营养/表没食子儿茶素没食子酸酯/生物合成/茶树Key words
Potassium and magnesium nutrition/Epigallocatechin gallate/Biosynthesis/Tea plant分类
农业科技引用本文复制引用
马骏杰,杨楠,林阳顺,高水练..钾镁营养对茶树EGCG生物合成调控作用研究[J].现代农业研究,2025,31(10):60-69,10.基金项目
福建日春实业有限公司泉州市特级人才创新实验室创新基金项目"钾肥改良茶园土壤酸化技术研究"(项目编号:RCTL2024001) （项目编号:RCTL2024001）
福建农林大学科技创新专项基金项目"钾镁改良茶园土壤酸化交互作用机理研究"(项目编号:KFB22109XA). （项目编号:KFB22109XA）
