上海海洋大学学报2026，Vol.35Issue(1)：1-12,12.DOI:10.12024/jsou.20241004656
凡纳滨对虾C型凝集素基因(LvCTLE)的克隆及其功能分析
Isolation and functional analysis of a C-type lectin gene(LvCTLE)in Litopenaeus vannamei
摘要
Abstract
C-type lectin(CTL)is an important pattern recognition receptor for recognizing carbohydrate structural domains.In this study we isolated and cloned a Litopenaeus vannamei C-type lectin,named LvCTLE,and analyzed its expression and function.The results of the study show that the full-length sequence of the LvCTLE gene cDNA was 1 231 bp with an open reading frame of 702 bp,encoding 233 amino acids.The LvCTLE amino acid sequence contained a signal peptide structure encoding 17 aa at the N-terminus and a sugar recognition structural domain encoding 134 aa at the C-terminus.The results showed that LvCTLE was distributed in the 9 tissues tested,with higher expression in muscle and gill tissues.After intramuscular injection of Vibrio parahaemolyticus,the expression of LvCTLE gene in both muscle and gill tissues of shrimp showed a tendency of up-regulation followed by a decrease,and the expression reached a peak at 12 h.Prokaryotically expressed recombinant rLvCTLE had Ca2+-dependent agglutination activity and could agglutinate Lactococcus garvieae,Escherichia coli,V.parahaemolyticus and V.tubiashii.D-glucose,D-galactose,D-mannose,D-fructose,maltose,sucrose and alginose all had inhibitory effects on the agglutination of rLvCTLE toward E.coli.These results suggested that LvCTLE could be involved in the immune response against bacterial challenge in the innate immune system of L.vannamei.关键词
凡纳滨对虾/C型凝集素/重组蛋白/凝集活性Key words
Litopenaeus vannamei/C-type lectin/recombinant protein/bacterial agglutination分类
农业科技引用本文复制引用
张欢,王瑶华,闫茂仓,冀德伟,黄贤克,罗奎,张敏..凡纳滨对虾C型凝集素基因(LvCTLE)的克隆及其功能分析[J].上海海洋大学学报,2026,35(1):1-12,12.基金项目
浙江省农业(水产新品种选育)新品种选育重大科技专项(2021C02069-5) （水产新品种选育）
浙江省重点研发计划(2021C02025) （2021C02025）
