青岛大学学报（医学版）2025，Vol.61Issue(6)：807-812,6.DOI:10.11712/jms.2096-5532.2025.61.110
依布硒在真菌性角膜炎中的体外抗真菌及抗炎作用
In vitro anti-fungal and anti-inflammatory effects of ebselen in fungal keratitis
摘要
Abstract
Objective To investigate the in vitro anti-fungal and anti-inflammatory effects of ebselen(EB)in fungal kerati-tis.Methods The minimal inhibitory concentration(MIC)of EB on Aspergillus fumigatus was determined.Calcium fluores-cent white(CFW)staining and propidium iodide(PI)staining were used to observe the effect of EB at various concentrations(0,8,16,32 mg/L)on the mycelial growth and cell membrane of A.fumigatus;HE staining was used to observe the ability of A.fumigatus spores to adhere to human corneal epithelial cells(HCECs);CCK-8 assay was used to observe the effect of different concentrations of EB on the viability of mouse macrophages(RAW264.7).Quantitative real-time PCR was used to measure the changes in the mRNA expression levels of the inflammatory cytokines IL-1β,IL-6 and TNF-α in RAW264.7 macrophages stimu-lated by inactivated A.fumigatus hyphae and treated by EB.Results MIC results showed that EB inhibited the growth of A.fumigatus.After 24 hours of culture,EB started to suppress A.fumigatus at a concentration of 4 mg/L in a concentration-de-pendent manner,and when the concentration of EB reached 32 mg/L or higher,it showed a significant inhibitory effect on A.fu-migatus which lasted to 48 hours(F=74.0-217.8,P＜0.001).CFW staining showed a gradual reduction in the density of A.fu-migatus hyphae with the increase in the concentration of EB,and PI staining showed that EB effectively disrupted fungal cell mem-branes.After EB treatment,there was a reduction in the number of spores adhering to the surface of HCECs(t=5.282,P＜0.001).EB solutions with a concentration of ≤64 mg/L did not affect the viability of RAW264.7 macrophages(P＞0.05).Further-more,EB inhibited A.fumigatus-induced inflammatory response in RAW264.7 macrophages and downregulated the mRNA ex-pression levels of IL-1β,IL-6,and TNF-α(F=100.9-168.3,P＜0.001).Conclusion EB can inhibit the growth of A.fu-migatus,reduce the adhesion ability of its cell membrane and spores,and suppress inflammatory response in macrophages induced by A.fumigatus.关键词
依布硒/角膜炎/眼感染,真菌性/烟曲霉菌/巨噬细胞/炎症Key words
ebselen/keratitis/eye infections,fungal/Aspergillus fumigatus/macrophages/inflammation分类
医药卫生引用本文复制引用
彭芳,刘星,吴瑗,贺铜,王桢涵,林静..依布硒在真菌性角膜炎中的体外抗真菌及抗炎作用[J].青岛大学学报（医学版）,2025,61(6):807-812,6.基金项目
山东省自然科学基金项目(ZR2023MH068) （ZR2023MH068）
