天津师范大学学报（自然科学版）2025，Vol.45Issue(6)：13-18,6.DOI:10.19638/j.issn1671-1114.20250603
米虾(Neocaridina davidi)CYP18A1基因克隆及其功能分析
Cloning and functional analysis of CYP18A1 gene in Neocaridina davidi
摘要
Abstract
To investigate the role of 26-hydroxylase(NhCYP18A1)in the molting and reproduction of Neocaridina davidi(shrimp),the full-length of NhCY P18A 1gene was cloned,and the expression levels of NhCY P18A 1at various developmental stages of shrimp individuals and during different gonadal developmental stage were assessed by qPCR.The molting rate of the shrimps and the gonadal index of the female shrimps were calculated,and the gonads of the shrimps were observed by longitudinal paraffin sections.The results demonstrated that:①The expression level of NhCYP18A 1was significantly higher in gonadally mature fe male shrimp compared to gonadal mature male shrimp;② During the gonadal development of female shrimp,the expression level of NhCY P18A 1 was significantly elevated in the middle and late stages compared to the early stages of gonadal development;③After successfully knocking down NhCY P18A 1,the cumulative molting rate of shrimp in the experimental group was significantly higher than that in the control group within 15 d,whereas the maturity and gonadal index of shrimp in the experimental group were notably lower than those in the control group.Based on these results,NhCY P18A 1 is involved in the processes of molting and gonadal development,primarily inhibiting molting and promoting go-nadal maturation in female shrimps.关键词
米虾/26-羟化酶(CYP18A1)/蜕皮/性腺发育/早熟/甲壳动物Key words
Neocaridina davidi/26-hydroxylase(CYP18A1)/molting/gonadal development/sexual precocity/Crustacea分类
生物科学引用本文复制引用
林然,周润林,周家露,李冉,孙金生..米虾(Neocaridina davidi)CYP18A1基因克隆及其功能分析[J].天津师范大学学报（自然科学版）,2025,45(6):13-18,6.基金项目
天津市科技重大专项与工程项目(24ZXZYSN00030) （24ZXZYSN00030）
天津市农业农村委员会水产种业创新攻关项目(SCZYGG202401). （SCZYGG202401）
