天津师范大学学报（自然科学版）2025，Vol.45Issue(6)：27-34,8.DOI:10.19638/j.issn1671-1114.20250605
ABA对大蒜愈伤组织中大蒜素含量的提升以及利用响应面法对大蒜素提取条件的优化
Promotion of allicin content in garlic callus by ABA and the optimization of allicin extraction conditions using response surface method
摘要
Abstract
To optimize the system for allicin production in callus and enhance the allicin content,different mass concentra-tions of exogenous abscisic acid(ABA)were supplemented into the callus induction and proliferation media to identify the optimal mass concentration for inducing allicin synthesis,using the storage leaves of Baodi garlic"Liu Ban Hong"as ex-plants.A single-factor analysis was conducted on five parameters in the production process(solid-liquid ratio,extraction time,ethanol volume fraction,enzymatic hydrolysis temperature and enzymatic hydrolysis time),the factors with greater in-fluence were screened according to the influence of different factors on the response value.On this basis,the allicin extrac-tion process was further optimized by response surface regression analysis.The results showed that:①The highest induction rate of callus tissue was 96.67%at ABA mass concentration of 0.10 mg/L,and the highest allicin content in the subcultured callus was 10.09 μg/g.②Three influential factors were identified through single factor analysis,namely the solid-liquid ra-tio,extraction time and ethanol volume fraction.③ The optimal system was obtained through response surface regression analysis,with a solid-liquid ratio of 1∶6,extraction time of 1.6 h,ethanol volume fraction of 80%,and the predicted con-tent of allicin extraction was 18.55 μg/g.④The callus allicin was extracted using the optimal system,and the verification value was 18.35 μg/g.The predicted value was basically consistent with the verification value,and the allicin content in-creased by 185.64%compared with the original method.关键词
大蒜/愈伤组织/大蒜素/脱落酸/响应面法Key words
garlic/callus/allicin/ABA/response surface method分类
农业科技引用本文复制引用
张艳婷,王玉雯,周诗瑶,高铭峰,王娅欣,范宝莉,王振英..ABA对大蒜愈伤组织中大蒜素含量的提升以及利用响应面法对大蒜素提取条件的优化[J].天津师范大学学报（自然科学版）,2025,45(6):27-34,8.基金项目
天津市农委科技成果转化与推广项目(202101060) （202101060）
天津市优秀农业科技特派员项目(22ZYCGSN00090,23ZYCGSN00270). （22ZYCGSN00090,23ZYCGSN00270）
