畜牧与兽医2026，Vol.58Issue(2)：40-47,8.
植物乳杆菌9-4的分离鉴定及其对小鼠结肠炎缓解作用的研究
Isolation and identification of Lactobacillus plantarum 9-4 and its effect on alleviating mice colitis
摘要
Abstract
To develop probiotic products capable of regulating oxidative stress and alleviating intestinal inflammation in animals,Lactobacil⁃lus plantarum strain 9-4 was isolated and screened from fresh wild boar feces through in vitro antioxidant tests.Antibacterial activity of the strain was detected via the Oxford cup method,and its tolerance to artificial gastrointestinal fluids and bile salts was evaluated.Then,a mouse colitis model was established using 3％dextran sulfate sodium(DSS),and the effects of L.plantarum 9-4 intervention on clinical symptoms,intestinal inflammation,and serum oxidative stress levels in colitis mice were analyzed.The results were as follows:L.plantarum 9-4 exhibited inhibitory effects on Escherichia coli,Salmonella,and Staphylococcus aureus,and maintained a high survival rate in artificial gastric juice,artificial intestinal fluid,and 0.3％bile salt.Intervention with L.plantarum 9-4 significantly increased the body weight of the colitis mice(P<0.001),reduced the disease activity index(DAI)(P<0.001)in the rodents,and attenuated their colon length shortening(P<0.05).Additionally,L.plantarum 9-4 intervention significantly decreased the serum malondialdehyde(MDA)levels in the mice(P<0.01),while the superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)levels in them showed an upward trend.Moreover,the mRNA expression levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in the colon tissue of the mice were significantly downregulated(P<0.05).In conclusion,L.plantarum 9-4 possessed antioxidant and anti- inflammatory effects and promised a potential to be developed into probiotic preparation for use in livestock and poultry farming.关键词
植物乳杆菌/肠道炎症/氧化应激/畜禽养殖Key words
Lactobacillus plantarum/intestinal inflammation/oxidative stress/livestock and poultry breeding分类
农业科技引用本文复制引用
高祎欣,贺晚秋,宋素泉..植物乳杆菌9-4的分离鉴定及其对小鼠结肠炎缓解作用的研究[J].畜牧与兽医,2026,58(2):40-47,8.基金项目
国家重点研发计划项目(2022YFC2105005) （2022YFC2105005）
