畜牧与兽医2026，Vol.58Issue(2)：56-60,5.
猪血浆中替米考星高效液相色谱检测方法的建立
Development of a high-performance liquid chromatography method for the quantification of tilmicosin in porcine plasma
摘要
Abstract
To develop a high-performance liquid chromatography(HPLC)method for quantifying tilmicosin in porcine plasma,tilmicosin standards were spiked into plasma samples obtained from healthy piglets,followed by extraction with methanol and chloroform.Chromato-graphic separation was achieved on an Agilent ZOR BAX Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm)under gradient elution con-ditions,with quantification performed via an external standard calibration curve.The results were that method validation exhibited excellent linearity(R2=0.999)across the concentration range of 0.15-50 μg/mL,as described by the regression equation y=9 956.3x-798.18.The intra-day and inter-day precision values were 0.30％-2.46％and 1.25％-4.78％,respectively,with recovery rates ranging from 85.37％to 118.45％.In conclusion,the above results indicated that the present method showed superior specificity,sensitivity and repro-ducibility,confirming its appropriate application to the accurate determination of tilmicosin in porcine plasma.关键词
替米考星/高效液相色谱/血浆/猪/外标法Key words
tilmicosin/HPLC/plasma/pigs/external standard method分类
农业科技引用本文复制引用
赵莎莎,郭大伟,程星星,龚佳豪,汪宇涵,赵源,司星星,张梦醒,魏经阁,高修歌..猪血浆中替米考星高效液相色谱检测方法的建立[J].畜牧与兽医,2026,58(2):56-60,5.基金项目
江苏农牧科技职业学院科技项目(NSF2024ZR03) （NSF2024ZR03）
