畜牧与兽医2026，Vol.58Issue(2)：71-79,9.
猪链球菌1型菌株的分离鉴定及生物学特性研究
Isolation,identification and biological characteristics of a Streptococcus suis serotype 1 strain
摘要
Abstract
To investigate the etiology of respiratory diseases in piglets from a swine farm in Guizhou Province and characterize the biological features of the causative pathogen,samples of lung tissues from dead pigs were aseptically collected for bacterial isolation.Suspected strains were purified via streaking on serum-enriched tryptone soybean agar(TSA)medium.The isolates were then systematically identified through morphological analysis,biochemical profiling,16S rRNA gene sequencing,andPCR-RFLP.Further characterization included virulence gene detection and antimicrobial susceptibility testing.Finally,pathogenicity was evaluated using a murine infection model.The results were that the isolated strain was designated as Streptococcus suis serotype 1(SS1)strain S240051,it was resistant to 8 antibiotics,including gentami-cin,kanamycin,neomycin,streptomycin,doxycycline,tetracycline,vancomycin and sulfamethoxazole,and it was sensitive to 11 antibiotics including penicillin,amoxicillin,cefotaxime,ampicillin,ciprofloxacin,enrofloxacin,levofloxacin,tiamulin,clindamycin and rifampin.Vir-ulence genotyping confirmed the presence of sly,epf,mrp,orf2,fbps,gapdh,strA,ccpA genes.When the infection concentration of the bac-terium was 3.96×109 CFU/mouse,100％of the mice died.Histopathological analysis revealed significant lesions in the lung,liver,and spleen tissues of the infected mice.This study enriched the pathogenic and phenotypic data on Streptococcus suis and presented foundational insights into the drug resistance mechanisms and pathogenicity of Streptococcus suis Serotype 1.关键词
猪链球菌1型/毒力基因/致病性/耐药性/分离鉴定Key words
Streptococcus suis serotype 1/virulence genes/pathogenicity/drug resistance/isolation and identification分类
农业科技引用本文复制引用
陈家丽,吴屿彤,周思旋,林如涛,史开志,吴昊,陈席敏,陈玉霞,王冬丁,朱祉聿..猪链球菌1型菌株的分离鉴定及生物学特性研究[J].畜牧与兽医,2026,58(2):71-79,9.基金项目
贵州省科技计划项目(黔科合支撑[2022]重点032,黔科合支撑[2022]一般088,黔农科科技创新[2023]07,黔农科院科技创新[2022]01,黔科合成果[2022]一般007号) （黔科合支撑[2022]重点032,黔科合支撑[2022]一般088,黔农科科技创新[2023]07,黔农科院科技创新[2022]01,黔科合成果[2022]一般007号）
