烟草科技2026，Vol.59Issue(1)：20-29,10.DOI:10.16135/j.issn1002-0861.2025.0505
林烟草与绒毛状烟草叶面表皮毛表型分化及分子基础的比较
Phenotypic differentiations of leaf trichome of Nicotiana sylvestris and Nicotiana tomentosiformis and their molecular basis
摘要
Abstract
To examine the differences in the type,secretion composition and genetic regulation mechanisms of leaf trichomes between Nicotiana sylvestris and Nicotiana tomentosiformis,the leaf trichomes were observed and counted by electron microscopy,the composition of glandular trichome secretions were analyzed by gas chromatography-mass spectrometry(GC-MS),and trichome development and the expression levels of glandular trichome secretions synthesis related genes were analyzed by real-time fluorescent quantitative PCR(qRT-PCR).The results showed that the leaf surface of N.tomentosiformis was mainly covered by protective trichomes and short stalk glandular trichomes,and long stalk glandular trichomes were rare.The leaf surface of N.sylvestris was mainly covered by long glandular trichomes.The content of cembranoid diterpenoids in the glandular trichome secretions of N.sylvestris was significantly higher than that of N.tomentosiformis,and sucrose esters were not detected in the glandular trichome secretions of the latter.The key genes associated with the synthesis of cembranoid diterpenoids,labdanoid diterpenoids and sucrose esters were highly expressed in N.sylvestris.The trichome development related genes NtARF3,NtCycB2,NtAquaporin,NtMIXTA and NtBCR2a were highly expressed in N.tomentosiformis,while NtHD1 and NtSAP1 were highly expressed in N.sylvestris.In summary,during the evolution of N.sylvestris and N.tomentosiformis,the trichome phenotypes were differentiated and different metabolic adaptative strategies were formed.The differences were determined by the expression patterns of key genes associated with the synthesis of glandular trichome secretion and the regulatory network of trichome genetic development.关键词
林烟草/绒毛状烟草/表皮毛/腺毛分泌物/基因表达Key words
Nicotiana sylvestris/Nicotiana tomentosiformis/Trichome/Glandular trichome secretion/Gene expression分类
农业科技引用本文复制引用
闫美琦,王琦,李玉娥,张健麒,梁珂馨,张世强..林烟草与绒毛状烟草叶面表皮毛表型分化及分子基础的比较[J].烟草科技,2026,59(1):20-29,10.基金项目
国家自然科学基金面上项目"转录因子NtHD9和NtHD12调控烟草长柄腺毛发生的分子机制研究"(32472104) （32472104）
福建省烟草公司南平市公司项目"基于转录组和代谢组联合分析翠碧一号上部叶褐变机理及采烤关键技术研究"(2024350700240143). （2024350700240143）
