烟草科技2026，Vol.59Issue(1)：30-36,7.DOI:10.16135/j.issn1002-0861.2025.0393
解淀粉芽孢杆菌ZY-9-13与携带病毒FpgMBV1假禾谷镰孢菌FC136-2A协同防治烟草黑胫病
Combined application of Bacillus amyloliquefaciens ZY-9-13 and Fusarium pseudograminearum FC136-2A carrying virus FpgMBV1 against tobacco black shank
摘要
Abstract
To clarify the control effect of combined application of Bacillus amyloliquefaciens ZY-9-13 and Fusarium pseudograminearum carrying virus FC136-2A on tobacco black shank and to determine their optimal ratio,the colony growth rate method was employed to analyze the toxicities of the two and their compounds at different ratios against Phytophthora nicotianae.Field trials were conducted to compare the control efficacies of single agents and their optimal compound against tobacco black shank.The results showed that the biocompatibility between Bacillus amyloliquefaciens ZY-9-13 and Fusarium pseudograminearum FC136-2A carrying FpgMBV1 was satisfactory and their effective median inhibitory concentrations against Phytophthora nicotianae were 3.3×105 CFUs per milliliter and 2.6×105 spores per milliliter,respectively.The compounds of Bacillus amyloliquefaciens ZY-9-13 and Fusarium pseudograminearum FC136-2A carrying FpgMBV1 with volume ratio ranged from 6∶4 to 4∶6 demonstrating synergistic inhibitory effects.Among which,the compound volume ratio of 5∶5 yielded a field control efficacy of 73.49%which was significantly higher than those of single agents,with the highest inhibition rate and it's increase rate and the strongest synergistic effect.关键词
烟草黑胫病/生物防治/解淀粉芽孢杆菌/假禾谷镰孢菌/真菌病毒/毒力Key words
Tobacco black shank/Biological control/Bacillus amyloliquefaciens/Fusarium pseudogra-minearum/Mycovirus/Toxicity分类
农业科技引用本文复制引用
薛超群,胡利伟,杨萌萌,王建伟,高飞,李建华,奚家勤..解淀粉芽孢杆菌ZY-9-13与携带病毒FpgMBV1假禾谷镰孢菌FC136-2A协同防治烟草黑胫病[J].烟草科技,2026,59(1):30-36,7.基金项目
河南省科技攻关项目"ZY-9-13与FpgMBV1防控烟草黑胫病的协同增效作用研究及应用"(242102110204). （242102110204）
