湖北科技学院学报（医学版）2026，Vol.40Issue(1)：6-11,6.DOI:10.16751/j.cnki.2095-4646.2025070815
新藤黄酸对喉癌细胞增殖、迁移的抑制作用及其机制研究
Inhibitory Effects of Gambogenic Acid on Proliferation and Migration of Laryngeal Cancer Cells and Its Mechanism
摘要
Abstract
Objective To investigate the effect of gambogenic acid on the proliferation and migration of laryngeal cancer Tu686 cells and its mechanism.Methods The effects of gambogenic acid on the growth and migration of Tu686 cells were analyzed by MTT assay,plate cloning assay,wound healing assay,Transwell assay and Hoechst 33258 staining.The molecular targets of gambogenic acid on laryngeal cancer cells was predicted by Network pharmacology and molecular docking,and the expression levels of related proteins were detected by Western blot.Results Gambogenic acid significantly inhibited the proliferation,migration and invasion of Tu686 cells.Network pharmacology screening identified 19 common targets of gambogenic acid-laryngeal cancer.KEGG enrichment analysis suggested that gambogenic acid might inhibit tumor progression by regulating cancer-related pathways.Molecular docking results showed that gambogenic acid had strong binding activity to the core targets.Hoechst 33258 staining and Western blot results showed that gambogenic acid could induce apoptosis of Tu686,down-regulate the expression of STAT3 and BCL2 proteins and up-regulate the expression of BAX protein.Conclusion Gambogenic acid can significantly inhibit the proliferation and migration of laryngeal cancer Tu686 cells,which may be related to multiple targets.关键词
新藤黄酸/喉癌细胞/分子对接/增殖/凋亡Key words
Gambogenic acid/Laryngeal cancer cells/Molecular docking/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
刘艳,谢孟婷,蔡敬,贾爱亭,宁志丰,朱宏儒..新藤黄酸对喉癌细胞增殖、迁移的抑制作用及其机制研究[J].湖北科技学院学报（医学版）,2026,40(1):6-11,6.基金项目
湖北科技学院五官专项(WGZX-02) （WGZX-02）
湖北省重点研发计划(大健康专项)(2022BCE011) （大健康专项）
