湖北科技学院学报（医学版）2026，Vol.40Issue(1)：12-16,28,6.DOI:10.16751/j.cnki.2095-4646.2025041710
荷叶多糖的酵母菌发酵提取及体外抗氧化活性研究
Study on Yeast Fermentation Extraction and In Vitro Antioxidant Activity of Lotus Leaf Polysaccharide
摘要
Abstract
Objective To optimize the fermentation conditions for extracting polysaccharides from lotus leaf powder and to evaluate the antioxidant activity of the obtained polysaccharides.Methods Using the yield of lotus leaf polysaccharide as the response value,the opti-mum fermentation conditions were determined by single factor experiments and response surface methodology.The scavenging rate of lotus leaf polysaccharide against 2,2-diphenyl-1-picrylhydrazyl(DPPH·)and hydroxyl radical(·OH)were determined by ultraviolet spectro-photometry.Results The optimal conditions for the extraction of lotus leaf polysaccharide by yeast fermentation were as follows:fermentation temperature 27.6℃,fermentation time 48 h,yeast addition 2.6%,and liquid-solid ratio 26mL/g.Under these conditions,the extraction rate of crude polysaccharide was 4.19%,with a polysaccharide content of 19.30%.The scavenging rates of DPPH·and·OH were 73.3%and 93.4%,respectively.Conclusion The yeast fermentation method can not only improve the extraction rate of lotus leaf polysac-charide,but also enhance its antioxidant activity,providing a reference for the industrial extraction of lotus leaf polysaccharide.关键词
荷叶/多糖/酵母菌/响应面优化/抗氧化活性Key words
Lotus leaf/Polysaccharide/Yeast/Response surface optimization/Antioxidant activity分类
医药卫生引用本文复制引用
曲晓营,邢宇畅,柴爽,朱晓明,刘小玲..荷叶多糖的酵母菌发酵提取及体外抗氧化活性研究[J].湖北科技学院学报（医学版）,2026,40(1):12-16,28,6.基金项目
国家自然科学基金(21972108) （21972108）
湖北省中央引导地方科技发展专项(2024CSA085) （2024CSA085）
湖北省教育厅科学技术研究项目(B2022184,BXLBX0780,S202110927039) （B2022184,BXLBX0780,S202110927039）
