畜牧与兽医2026，Vol.58Issue(2)：1-10,10.
拜城油鸡不同生长阶段脂肪代谢相关基因表达量与脂肪性状的相关性研究
Correlation between fat metabolism-related gene expression and fat traits in different growth stages of Baicheng fatty chicken
摘要
Abstract
The aim of this study was to explore the relationship between intestinal the fatty acid binding protein(I-FABP),apolipoprotein A1(APOA1),microsomal triglyceride transfer protein(MTTP),microsomal triglyceride transfer protein-like(MTTPL),adipose differ-entiation-related protein(ADFP)and peroxisome proliferator-activated receptor γ(PPARγ)genes and the fat traits in Baicheng fatty chickens at different growth and development stages,and to lay a foundation for further study on the role of candidate genes in fat traits.A to-tal of 300 samples of pectoral muscle,leg muscle,liver,abdominal fat,back fat and chest fat were collected from Baicheng fatty chickens at different ages(14,28,56,84,112 days),and their fat traits and blood lipid indexes were determined.Real-time quantitative PCR was used to detect the expression levels of the I-FABP,APOA1,MTTP,MTTPL,ADFP and PPARγ genes in different tissues of the Baicheng fatty chickens at different growth stages.The results showed that the expression of the I-FABP gene was the highest in the liver at 14 days of age,the expression of the PPARγ gene was the highest in the liver at 112 days of age,the APOA1 and MTTPL genes were highly expressed in liver and muscle,the MTTP gene was highly expressed in abdominal fat,and the ADFP gene was highly expressed in chest fat.The corre-lation analysis showed that the expression of the I-FABP gene was significantly positively correlated with the intramuscular fat(IMF)of the pectoral muscle and leg muscle of the chickens(P<0.01),and significantly negatively correlated with the chest fat weight(P<0.05).The expression of the I-FABP gene in the abdominal fat,back fat and chest fat was significantly positively correlated with low density lipoprotein(LDL)(P<0.05).The expression of the MTTP gene in liver was significantly negatively correlated with triglyceride(TG)(P<0.05),and its expression in the chest fat was significantly negatively correlated with LDL(P<0.05).The expression of the MTTPL gene was significant-ly negatively correlated with the leg muscle IMF(P<0.05),and its expression in the abdominal fat was significantly positively correlated with high density lipoprotein(HDL)(P<0.01).The expression of the PPARγ gene in the back fat was significantly positively correlated with LDL(P<0.05).In conclusion,the I-FABP,MTTP,MTTPL and PPARγ genes played an important role in the formation of fat traits in Baicheng fatty chickens.关键词
拜城油鸡/组织/脂肪性状/候选基因/基因表达Key words
Baicheng fatty chicken/tissue/fat trait/candidate gene/gene expression分类
农业科技引用本文复制引用
萧蓉,姜薇薇,王局东,张海月,孙杰..拜城油鸡不同生长阶段脂肪代谢相关基因表达量与脂肪性状的相关性研究[J].畜牧与兽医,2026,58(2):1-10,10.基金项目
国家自然科学基金项目(31660654) （31660654）
石河子大学大学生研究训练计划项目(SRP2024020) （SRP2024020）
