湖北科技学院学报（医学版）2026，Vol.40Issue(1)：17-22,6.DOI:10.16751/j.cnki.2095-4646.2025050701
基于网络药理学和细胞实验探究青藤碱抗衰老的作用机制
Investigating the Anti-Aging Mechanism of Sinomenine Using Network Pharmacology and Cell Experiments
摘要
Abstract
Objective To explore the effect of sinomenine(SIN)on D-galactose-induced macrophage senescence by using network pharmacology and cell experiments.Methods Data sources such as PubChem,SwissTargetPrediction,PharmMapper,and GeneCards were used to obtain common genes related to sinomenine and senescence.GO and KEGG pathway enrichment analyses were performed using the DAVID database and the Microbial Information Platform.A compound-target network was constructed using Cytoscape 3.10.2,and a protein-protein interaction(PPI)network was plotted to identify core genes.RAW264.7 cells were cultured in vitro and divided into three groups:the control group,the D-galactose-induced senescence model group,and the D-galactose+sinomenine group.The effect of sinomenine on RAW264.7 cell senescence was evaluated by β-galactosidase staining associated with senescence,and Western blot was used to detect chan-ges in p53 and p-AKT1/AKT1 protein expression.Results The enrichment analysis involved a total of 914 signaling pathways,541 biological processes,144 molecular functions,and 73 cellular components,mainly focusing on pathways related to cancer pathogenesis,and the PI3K-AKT signaling pathway in SIN therapy for aging.Compared with the normal control group,the proportion of β-galactosidase-positive cells and the expression of p53 protein were increased,while the expression of p-AKT1/AKT1 protein was decreased in the senescence model group(all P＜0.05).Compared with the senescence model group,the proportion of β-galactosidase-positive cells and the expression of p53 pro-tein were decreased,while the expression of p-AKT1/AKT1 protein was increased in the D-galactose+sinomenine group(all P＜0.05).Conclusion Sinomenine can delay the senescence of RAW264.7 macrophages induced by D-galactose by regulating p-AKT1/AKT1 signa-ling pathway.关键词
RAW264.7/衰老/青藤碱/网络药理学/D-半乳糖Key words
RAW 264.7/Aging/Sinomenine/Network pharmacology/D-galactose分类
医药卫生引用本文复制引用
付晓俐,肖嘉琪,潘欣仪,李朋坤,全羽欣,包永芬,瞿利花,单士刚..基于网络药理学和细胞实验探究青藤碱抗衰老的作用机制[J].湖北科技学院学报（医学版）,2026,40(1):17-22,6.基金项目
湖北科技学院糖尿病心脑血管病变湖北省重点实验室开放基金项目(2020TNB07) （2020TNB07）
