湖北科技学院学报（医学版）2026，Vol.40Issue(1)：34-38,43,6.DOI:10.16751/j.cnki.2095-4646.2025072001
虾青素缓解草甘膦诱导的小鼠脑神经损伤
Astaxanthin Alleviates Brain Neuroinjury Induced by Glyphosate in Mice
摘要
Abstract
Objective To investigate the alleviating effect of astaxanthin(AST)on glyphosate(GLY)-induced brain neuroinjury in mice via tail vein injection and its underlying mechanisms.Methods KMmale mice were randomly assigned into a control group(CON),a GLY exposure group(1 mg/kg via tail vein injection),and a GLY exposure+astaxanthin treatment group(GLY+AST,administered 50 mg/kg AST via gavage following GLY exposure).Continuous administration lasted for 35 days.On day 27 of administration,Morris water maze,open field,and elevated plus maze tests were conducted to assess learning and memory as well as anxiety-like behaviors.On day 36,brain tissues were collected.Nissl staining was employed to observe neuronal morphological changes.Molecular docking technology was uti-lized to analyze the binding activities of GLY and AST with β-site amyloid precursor protein cleaving enzyme 1(BACE1).Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of BACE1,and β-amyloid proteins Aβ1-40 and AβAβ1-42 in brain tissue.Results Behavioral tests revealed that GLY exposure significantly impaired spatial learning and memory,and increased anxiety-like behaviors in mice.Nissl staining showed disordered neuronal arrangement,reduced Nissl bodies,and an increased proportion of necrotic cells in the hip-pocampus and other brain regions of the GLY group.Molecular docking results demonstrated that both GLY and AST could effectively bind to BACE1,with binding energies of-6.3 kcal/mol and-8.3 kcal/mol,respectively.ELISA detection indicated that the levels of BACE1,Aβ1-40,and AβAβ1-42 in the brain tissue of the GLY group were significantly higher compared to the CON group(all P＜0.05).AST inter-vention significantly ameliorated the behavioral impairments(P＜0.05),alleviated neuronal damage,reduced the proportion of necrotic cells,and effectively suppressed the increases in BACE1,Aβ1-40,and Aβ1-42 levels induced by GLY exposure.Conclusion GLY exposure via tail vein injection can induce brain neuroinjury and cognitive dysfunction in mice,potentially involving the activation of the BACE1/Aβpath-way.AST can effectively alleviate GLY-induced brain neuroinjury by suppressing BACE1 expression and Aβgeneration.关键词
虾青素/草甘膦/神经行为学/β-位点淀粉样前体蛋白裂解酶1Key words
Astaxanthin/Glyphosate/Neurobehavioral assessment/BACE1分类
医药卫生引用本文复制引用
马亚萍,张晓琳,王贝贝,徐旭龙,晏彪,佘同辉..虾青素缓解草甘膦诱导的小鼠脑神经损伤[J].湖北科技学院学报（医学版）,2026,40(1):34-38,43,6.基金项目
湖北科技学院五官医学院专项科研基金(2020WG09) （2020WG09）
湖北科技学院横向项目(2024HX139) （2024HX139）
