湖北科技学院学报（医学版）2026，Vol.40Issue(1)：39-43,5.DOI:10.16751/j.cnki.2095-4646.2025070702
SFRP1对结直肠癌细胞增殖和侵袭的影响及其机制研究
Effects of SFRP1 on Colorectal Cancer Cell Proliferation and Invasion and Its Underlying Mechanisms
摘要
Abstract
Objective To investigate the effects of Secreted Frizzled-Related Protein(SFRP1)on the proliferation,invasion,and mi-gration of colorectal cancer cells and its underlying mechanisms,providing a new research direction for clinical treatment.Methods Bioinfor-matics analysis was used to study the expression level of SFRP1 in colorectal cancer and its correlation with colorectal cancer invasion and proliferation,investigating the relationship between colorectal cancer and SFRP1.SW480 cells were transfected with SFRP1 inhibitor(small interfering RNA)and negtive control(si-NC).CCK-8 colorimetric assay and Transwell assay were performed to evaluate the proliferation,in-vasion and migration abilities of each group cells.Western blot analysis was used to determine the protein levels of Wnt4 and β-catenin,key components of the Wnt/β-catenin signaling pathway,in each group of cells.Results The expression of SFRP1 protein was down-regulated in colorectal cancer cells(P＜0.05).Compared with the control group,the proliferation,invasion and migration ability of si-SFRP1 group en-hanced,the expression of Wnt4 and β-catenin protein expressione elevated(P＜0.05).Conclusion Downregulation of SFRP1 expression may promote the development and progression of colorectal cancer by regulating the Wnt/β-catenin signaling pathway,providing new insights into the clinical treatment of colorectal cancer.关键词
分泌型卷曲相关蛋白1/结直肠癌/Wnt/β-catenin信号转导通路/肿瘤转移Key words
SFRP1/Colorectal cancer/Wnt/β-catenin signaling pathway/Tumor metastasis分类
医药卫生引用本文复制引用
熊诗颖,杨振,邵文驭,李成武,司马学琴..SFRP1对结直肠癌细胞增殖和侵袭的影响及其机制研究[J].湖北科技学院学报（医学版）,2026,40(1):39-43,5.基金项目
湖北省大学生创新训练项目(S202410927039) （S202410927039）
湖北科技学院横向项目(2024HX084) （2024HX084）
