湖北科技学院学报（医学版）2026，Vol.40Issue(1)：44-48,5.DOI:10.16751/j.cnki.2095-4646.2025042916
SH3 BGRL的表达对AngⅡ诱导的H9C2心肌细胞肥大的影响
Effect of SH3 BGRL Expression on AngⅡ-Induced Hypertrophy in H9 C2 Cardiomyocytes
摘要
Abstract
Objective To investigate the effect of SH3 domain-binding glutamate-rich protein-like protein(SH3BGRL)expression on cardiomyocyte hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ).Methods A hypertrophy model of H9C2 cardiomyocytes was construc-ted.Western blot and RT-PCR were used to detect the expression level of SH3BGRL,and RT-PCR was used to detect the expression of car-diac hypertrophy-related marker genes ANP,BNP,and β-MHC.Results The mRNA expression levels of cardiac hypertrophy-related marker genes significantly increased after overexpression of SH3BGRL(P＜0.05).Conversely,the mRNA expression levels of cardiac hypertrophy-related marker genes significantly decreased after silencing SH3BGRL(P＜0.05).Conclusion The expression level of SH3BGRL is posi-tively correlated with the expression of cardiac hypertrophy marker genes,suggesting that it could be a potential target for treating myocardial hypertrophy and laying a solid foundation for further research into the function of SH3BGRL in the heart.关键词
SH3域结合谷氨酸丰富蛋白样蛋白/心肌肥大/转染/H9C2细胞Key words
SH3BGRL/Cardiac hypertrophy/Transfection/H9C2 cells分类
医药卫生引用本文复制引用
高润泽,魏小茗,韩婉婷,苏莹,彭铁,朱丹..SH3 BGRL的表达对AngⅡ诱导的H9C2心肌细胞肥大的影响[J].湖北科技学院学报（医学版）,2026,40(1):44-48,5.基金项目
湖北省自然科学基金(2024AFB1025) （2024AFB1025）
湖北科技学院博士启动基金(BK202414) （BK202414）
湖北科技学院医学科研专项基金(2022YKY18) （2022YKY18）
