现代中西医结合杂志2025，Vol.34Issue(22)：3102-3109,8.DOI:10.3969/j.issn.1008-8849.2025.22.006
桂仙合剂粪菌移植干预绝经综合征潮热的作用机制研究
Mechanism of Guizhi and Erxian decoction in the treatment of menopausal syndrome-vasomotor symptoms through fecal microbiota transplantation
摘要
Abstract
Objective It is to explore the mechanism of Guizhi and Erxian decoction(GED)in the treatment of meno-pausal syndrome-vasomotor symptoms(MPS-VMS)through fecal microbiota transplantation based on urine metabolomics and gut microbiota.Methods Forty-eight female SD rats were randomly divided into the following groups:healthy group,VMS group,GED group,Climen group,healthy transplantion group,VMS transplantion group,GED transplantion group,and Climen transplantion group,with 6 rats in each group.The rats in the healthy group,healthy transplantion group,and VMS transplantion group were modeled with sham surgery,while the rats in the remaining groups underwent ovariectomy to establish models of MPS-VMS.After successful modeling was confirmed in 10 days after surgery,the healthy group and VMS group was given physiological saline via gavage;the GED group was given GED compound solution 33.3 g/kg by ga-vage;the Climen group was given estradiol valerate solution 0.4 mg/kg by gavage for the first 3 days,followed by estradiol valerate combined with cyproterone acetate solution 0.6 mg/kg by gavage for the subsequent 2 days.All the transplantion groups were treated with antibiotic cocktail therapy from 10th to14th day after surgery to achieve relative intestinal sterility.Subsequently,the healthy transplantion group was given fecal microbiota solution from healthy individuals by gavage,the VMS transplantion group was given fecal microbiota solution from VMS patients by gavage,the GED transplantion group was given fecal microbiota solution from MPS-VMS patients treated with GED by gavage,while the Climen transplantion group was given fecal microbiota solution from MPS-VMS patients treated with Climen by gavage.The urinary metabolite profiles of rats in each group were detected,and differential metabolites were identified,and metabolic pathway enrichment analysis was performed;fecal samples was collected for 16S rRNA gene sequencing,followed by bioinformatics analysis.Results GED primarily influenced 17 biomarkers by regulating gut microbiota.These biomarkers were associated with 5 metabolic pathways:phosphonate and phosphinate metabolism,porphyrin metabolism,glycine-serine-threonine metabolism,purine metabolism,and steroid hormone biosynthesis.The differential metabolites significantly influence these pathways included ciliatine,5-aminolevulinic acid,2-pyrrolidine-glycine,xanthine and testosterone.The differential microbial genera were Clostridium baratii,Dorea,Actinomyces,and Phascolarctobacterium,in which Clostridium baratii and Phascolarctobacteri-um were positively correlated with xanthine and negatively correlated with 2-pyrrolidine-glycine.Conclusion GED fecal mi-crobiota transplantation may exert therapeutic effects on MPS-VSMS by regulating the"gut microbiota-metabolism"axis.关键词
桂仙合剂/绝经综合征潮热/粪菌移植/尿液代谢组学Key words
Guizhi and Erxian decoction/menopausal syndrome with vasomotor symptoms/fecal microbiota transplan-tation/urine metabolomics分类
医药卫生引用本文复制引用
赵婧冰,郭永,黄磊,陈思阳,宗云,周英..桂仙合剂粪菌移植干预绝经综合征潮热的作用机制研究[J].现代中西医结合杂志,2025,34(22):3102-3109,8.基金项目
广东省基础与应用基础研究基金企业联合基金项目(2023A1515220027) （2023A1515220027）
