中国免疫学杂志2026，Vol.42Issue(1)：20-27,8.DOI:10.3969/j.issn.1000-484X.2026.01.003
耐受性树突状细胞对胶原诱导性关节炎大鼠肠道菌群结构的影响
Effect of tolerogenic dendritic cells on gut microbiota structure in collagen-induced arthritis rats
摘要
Abstract
Objective:To investigate effect of tolerogenic dendritic cells(tolDC)on gut microbiota structure of rats with colla-gen-induced arthritis(CIA).Methods:Experimental animals were randomly divided into Control group,CIA group and tolDC group.CIA group and tolDC group were immunized with bovine type Ⅱ collagen solution to establish CIA model.TolDC group was treated with tolDC via tail vein on 21th day after initial immunization.Arthritis score and thickness of paws in each group of rats were mea-sured weekly.On the 35th day,rats were sacrificed and specimens were taken for testing.HE staining was performed on ankle joint to evaluate pathological changes in each group of rats.16S rDNA sequencing was used to detect changes in gut microbiota structure of rats in each group,including alpha diversity,beta diversity,species composition and species difference analysis.Results:Compared with Control group,arthritis score and thickness of paws in CIA group were increased,exhibited abnormal proliferation and extensive infiltration of inflammatory cells,as well as formation of pannus and destruction of bone and cartilage,intestinal flora index of Chao1 and Shannon were decreased,but difference was not significant,abundance of Firmicutes was decreased,abundance of Clostridioides and Muribaculum were increased.After tolDC treatment,arthritis score and thickness of paws of CIA rats were decreased,infiltration of inflammatory cells,abnormal hyperplasia of synovial tissue,formation of pannus and injury of bone and cartilage were alleviated,intestinal flora index of Chao1 was decreased(P<0.01),abundance of Firmicutes,Lactobacillus,Lachnoclostridium,GCA-900066575,Dubosiella,Peptococcus,Acetatifactor and Butyricicoccus were increased.Conclusion:TolDC alleviates joint injury in CIA rats and influences gut microbiota structure.关键词
耐受性树突状细胞/肠道菌群/类风湿关节炎/胶原诱导性关节炎Key words
Tolerogenic dendritic cells/Gut microbiota/Rheumatoid arthritis/Collagen-induced arthritis分类
医药卫生引用本文复制引用
周燕,万秀方,袁锐,包伦敏,李红红,郑婷,蒋红梅..耐受性树突状细胞对胶原诱导性关节炎大鼠肠道菌群结构的影响[J].中国免疫学杂志,2026,42(1):20-27,8.基金项目
国家自然科学基金地区科学基金(82360423) （82360423）
贵州省教育厅创新群体项目(黔教合KY字[2021]016) （黔教合KY字[2021]016）
贵州医科大学附属医院国家自然科学基金(NSFC)青年基金培育计划项目(gyfynsfc[2023]-14) （NSFC）
贵州省科技计划项目(黔科合基础-ZK[2024]一般193). （黔科合基础-ZK[2024]一般193）
