中国免疫学杂志2026，Vol.42Issue(1)：35-40,6.DOI:10.3969/j.issn.1000-484X.2026.01.005
积雪草苷调节RANKL/RANK/OPG信号通路对类风湿关节炎大鼠骨代谢和骨密度的影响
Effects of Asiaticoside on bone metabolism and bone density in rheumatoid arthritis rats by regulating RANKL/RANK/OPG signaling pathway
摘要
Abstract
Objective:To investigate effects of Asiaticoside(ASI)on bone metabolism,bone density and receptor activator of nuclear factor-κB ligand(RANKL)/receptor activator of nuclear factor-κB(RANK)/osteoprotegerin(OPG)signaling pathway in rheu-matoid arthritis(RA)rats.Methods:A RA rat model was constructed and randomly grouped into Model group,low and high doses ASI groups(ASI-L,ASI-H groups),OPG group and high-dose ASI+OPG group(ASI-H+OPG group),with 12 rats in each group.Another 12 healthy rats were included as control group.All rats were subjected to bone density analysis.Micro CT was used to detect bone microstructure.ELISA was applied to detect serum bone metabolism indicators Runx2,OC,ALP,CTX and inflammatory factor indicators IL-1β,TNF-α and IL-6 levels.Joint tissue lesions were observed by HE staining.Western blot was applied to detect expres-sions of RANKL,RANK and OPG proteins.Results:Compared with Control group,Model group showed severe cartilage tissue dam-age,extensive infiltration of inflammatory cells,number of osteoclasts was increased,bone mineral density(BMD)and bone metabo-lism indicators Runx2,OC levels,Tb.Th,Tb.N,BV/TV and Conn.D indexes were obviously decreased,levels of Tb.SP,SMI,ALP,CTX,and inflammatory factors IL-1β,TNF-α and IL-6 were obviously increased,expressions of RANKL and RANK proteins were obviously increased,expression of OPG protein was prominently decreased(P<0.05).Compared with Model group,with increase of ASI dose,degree of cartilage tissue damage,inflammatory cell infiltration,number of osteoclasts reduced in ASI-L and ASI-H groups,BMD and bone metabolism indicators Runx2,OC levels,Tb.Th,Tb.N,BV/TV,Conn.D indexes were prominently increased,while levels of Tb.SP,SMI,ALP,CTX,inflammatory factors IL-1β,TNF-α and IL-6 were obviously decreased,expres-sions of RANKL and RANK proteins were obviously decreased,and expression of OPG protein was prominently increased(P<0.05).Compared with ASI-H group,ASI-H+OPG group showed better improvement in cartilage tissue damage and inflammatory symptoms,number of osteoclasts decreased,BMD and bone metabolism levels were closer to Control group,expressions of RANKL and RANK proteins were prominently reduced,while expression of OPG protein was obviously increased(P<0.05).Conclusion:ASI can improve bone density and metabolism,reduce inflammatory response,and improve joint tissue damage in rats,whose potential mechanism may be related to regulation of RANKL/RANK/OPG signaling pathway.关键词
积雪草苷/类风湿关节炎/骨密度/骨代谢/RANKL/RANK/OPG通路Key words
Asiaticoside/Rheumatoid arthritis/Bone density/Bone metabolism/RANKL/RANK/OPG pathway分类
医药卫生引用本文复制引用
张航,熊成欢,唐芳..积雪草苷调节RANKL/RANK/OPG信号通路对类风湿关节炎大鼠骨代谢和骨密度的影响[J].中国免疫学杂志,2026,42(1):35-40,6.基金项目
国家自然科学基金项目(82160917). （82160917）
