中国免疫学杂志2026，Vol.42Issue(1)：53-57,5.DOI:10.3969/j.issn.1000-484X.2026.01.008
齐墩果酸调节IL-6/JAK2/STAT3信号通路对银屑病大鼠皮肤损伤的影响
Effect of oleanolic acid on skin injury in psoriasis rats by regulating IL-6/JAK2/STAT3 signaling pathway
摘要
Abstract
Objective:To investigate effect of oleanolic acid(OA)on skin injury in psoriasis(PsO)rats by regulating IL-6/Janus kinase 2(JAK2)/signal transducer and activator of transcription-3(STAT3)signaling pathway.Methods:Rats were separated into control group,PsO group,low,medium and high-dose OA groups(OA-L,OA-M,OA-H)and rIL-6 group(IL-6/JAK2/STAT3 signaling pathway activator).Except for control group,all other rats were induced into PsO models by imiquimod cream.After drug intervention,local skin injury was observed,and severity index PASI score of rat PsO area was evaluated.HE staining was applied to observe pathological changes in skin.ELISA was applied to detect levels of TNF-α and IL-6 in serum.qRT-PCR was applied to detect mRNA expressions of IL-6,JAK2 and STAT3.Western blot was applied to detect expressions of IL-6/JAK2/STAT3 pathway proteins.Results:Compared with control group,PASI score,epidermal thickness,inflammatory cell infiltration score,TNF-α,IL-6 levels,IL-6,JAK2,STAT3 mRNA,and IL-6,p-JAK2/JAK2,p-STAT3/STAT3 protein expressions in PsO group increased(P<0.05).Com-pared to PsO group,PASI score,epidermal thickness,inflammatory cell infiltration score,TNF-α,IL-6 levels,IL-6,JAK2,STAT3 mRNA,and IL-6,p-JAK2/JAK2,p-STAT3/STAT3 protein expressions were reduced in OA-L,OA-M and OA-H groups,and showed concentration dependence(P<0.05).IL-6/JAK2/STAT3 signaling pathway activator rIL-6 obviously reduced improvement effect of OA on skin injury in PsO rats(P<0.05).Conclusion:OA has effects of inhibiting inflammatory response and improving skin injury in PsO rats,and its mechanism is related to inhibiting activation of IL-6/JAK2/STAT3 signaling pathway.关键词
齐墩果酸/IL-6/Janus蛋白酪氨酸激酶2/信号转导与转录激活子3/银屑病/皮肤损伤Key words
Oleanolic acid/IL-6/Janus kinase 2/Signal transducer and activator of transcription-3/Psoriasis/Skin injury分类
医药卫生引用本文复制引用
周洋,李星汇,曹丽华,刘玉..齐墩果酸调节IL-6/JAK2/STAT3信号通路对银屑病大鼠皮肤损伤的影响[J].中国免疫学杂志,2026,42(1):53-57,5.基金项目
徐州医科大学附属医院发展基金资助项目(XY-FM202303). （XY-FM202303）
