中国免疫学杂志2026，Vol.42Issue(1)：58-64,7.DOI:10.3969/j.issn.1000-484X.2026.01.009
桔梗皂苷D通过Nrf2和NF-κB抑制LPS诱导星形胶质细胞的氧化应激和神经炎症
Platycodin D inhibits LPS induced oxidative stress and neuroinflammation in astrocytes through Nrf2 and NF-κB
摘要
Abstract
Objective:To explore the effects of platycodin D on lipopolysaccharide(LPS)induced astrocyte oxidative stress and cellular inflammatory response,as well as roles of Nrf2 and NF-κB pathways.Methods:Isolation and identification of astrocytes from the certex of 2-day old Wistar rats.With 1 μmol/L LPS induced astrocytes to establish a cellular inflammatory model,which was divided into control group,LPS group,and LPS+platycodon D(1,10,100 μmol/L)groups.Cell survival rate was measured by MTT assay,NO content was measured using Griess reaction,lipid peroxide malondialdehyde(MDA)and superoxide dismutase(SOD)contents were detected by reagent kits.Western blot was used to detect expression levels of cyclooxygenase-2(COX-2),inducible nitric oxide synthase(iNOS),IL-1β,IL-17,chemokine ligand 2(CCL2),chemokine C-X-C motif ligand 13(CXCL13),Nrf2,kelch like ECH associated protein 1(Keap1),NF-κB1,NF-κB2,IKBκB and V-rel reticuloendothelial virus oncogene homolog A(RELA).Results:LPS induced astrocytes showed a decrease in survival rate,an increase in NO production,an increase in MDA content,a decrease in SOD activity,up-regulation of COX-2,iNOS,IL-1β,IL-17,CCL2,CXCL13,Keap1,NF-κB1,NF-κB2,IKBκB and RELA expressions,while down-regulation of Nrf2 expression(P<0.05).Survival rate of astrocytes induced by LPS pretreatment with different concentrations of platycodon D(1,10 and 100 μmol/L)was increased,with less NO production,decreased MDA content,increased SOD activity,down-regulated expressions of COX-2,iNOS,IL-1β,IL-17,CCL2,CXCL13,Keap1,NF-κB1,NF-κB2,IKBκB and RELA,while up-regulated expression of Nrf2(P<0.05).Conclusion:Platycodin D has inhibitory effects on LPS induced oxidative stress and neuroinflammation in astrocytes,and exerts its effects through Nrf2 and NF-κB signaling.Platycodin D is a potential drug for development of neuroimmune related disease treatments and prevention.关键词
桔梗皂苷D/星形胶质细胞/Nrf2/NF-κB/氧化应激/炎症Key words
Platycodin D/Astrocytes/Nrf2/NF-κB/Oxidative stress/Inflammation分类
医药卫生引用本文复制引用
金艳,傅增辉,王春影,林再红,姜岩..桔梗皂苷D通过Nrf2和NF-κB抑制LPS诱导星形胶质细胞的氧化应激和神经炎症[J].中国免疫学杂志,2026,42(1):58-64,7.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(2022-KYYWF-0817). （2022-KYYWF-0817）
