中国临床药理学与治疗学2025，Vol.30Issue(12)：1640-1647,8.DOI:10.12092/j.issn.1009-2501.2025.12.007
负载Mg2+/槲皮素海藻酸钠水凝胶通过抑制中性粒细胞炎症反应和氧化应激发挥免疫调节功能
Mg2+/quercetin-loaded alginate hydrogel exerts immunomodulatory effects by suppressing neutrophil inflammation and oxidative stress
摘要
Abstract
AIM:To overcome the limitations of sodium alginate hydrogel(SAH),including its slow degradation and limited bioactivity in wound heal-ing applications,we developed a functionalized hy-drogel(SAQM)incorporating Mg²⁺ and quercetin(Que).We further conducted a systematic evalua-tion of its roles and underlying molecular mecha-nisms in modulating neutrophil-mediated inflam-matory responses and oxidative stress.METHODS:SAH and SAQM were prepared via ionic crosslink-ing induced by CaCl2.Morphology was character-ized by SEM,mechanical properties were analyzed via rheometry,and component release was quanti-fied using ICP-AES/UV spectrophotometry.In vitro evaluation employed a dHL60 neutrophil model stimulated with LPS.Inflammatory markers and NF-kB pathway activation were assessed by RT-qPCR and Western blot,while ROS generation was mea-sured via flow cytometry.RESULTS:In vitroexperi-ments demonstrated sustained release of Mg2+and Que from SAQM.SEM and rheological analyses re-vealed comparable gelation and microstructure be-tween SAQM and SAH,but SAQM showed signifi-cantly reduced swelling and enhanced degradation rates.Biocompatibility studies confirmed negligible cytotoxicity toward neutrophils.In LPS-induced in-flammation,SAQM markedly suppressed pro-in-flammatory cytokine expression and ROS produc-tion.Compositional analysis indicated Mg2+primari-ly mediated anti-inflammatory effects,while Que contributed to antioxidant activity.Notably,SAQM treatment inhibited LPS-induced activation of the canonical NF-kB pathway,suggesting its anti-inflam-matory mechanism involves NF-kB suppression.CONCLUSION:SAQM combines improved degrad-ability with dual anti-inflammatory and antioxidant properties,representing a promising immunomodu-latory hydrogel dressing for clinical applications.关键词
Mg2+/槲皮素/海藻酸钠/中性粒细胞/抗炎抗氧化Key words
Mg2+/quercetin/sodium alginate/neutrophil/anti-inflammation/antioxidation分类
医药卫生引用本文复制引用
杨帆,金亮,陈路阳,李兰,周海峰,吴静..负载Mg2+/槲皮素海藻酸钠水凝胶通过抑制中性粒细胞炎症反应和氧化应激发挥免疫调节功能[J].中国临床药理学与治疗学,2025,30(12):1640-1647,8.基金项目
浙江省中医药科技项目(2023ZF157,2025ZRZR017) （2023ZF157,2025ZRZR017）
国家自然科学基金(82304761) （82304761）
国家博士后资助计划(GZC20231701) （GZC20231701）
浦东新区科技发展基金事业单位民生科研专项项目(PKJ2024-Y16) （PKJ2024-Y16）
