中国临床药理学与治疗学2025，Vol.30Issue(12)：1648-1657,10.DOI:10.12092/j.issn.1009-2501.2025.12.008
基于TLR4/MyD88/NF-κB信号通路探讨过敏煎对特应性皮炎小鼠的保护机制
Protective mechanism of Guomin decoction against atopic dermatitis in mice based on TLR4/MyD88/NF-κB signaling pathway
摘要
Abstract
AIM:To investigate the protective ef-fect and mechanism of Guomin decoction on 1-chlo-ro-2,4-dinitrochlorobenzene(DNCB)induced atopic dermatitis(AD)in mice.METHODS:Fifty mice were induced by DNCB to establish atopic dermatitis mod-el,and were randomly divided into model group,Ce-tirizine hydrochloride group(1.3 mg/kg),Guomin decoction low,medium and high-dose groups(3.25,6.5,13 g/kg),and blank group 10 mice,60 mice in to-tal.Each group of mice was given the corresponding test drug according to the set dose by intragastric ad-ministration for 14 consecutive days.The skin le-sions were observed and the severity of skin lesions were scored.The organ index was calculated after treatment.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of skin le-sions.Serum immunoglobulin E(Ig-E),interleukin-6(IL-6),IL-1β,tumor necrosis factor alpha(TNF-ɑ),in-terferon gamma(IFN-γ)and transforming growth factor β(TGF-β)were detected by enzyme-linked im-munosorbent assay(ELISA).Real-time fluorescence quantitative polymerase chain reaction(Real-Time)the gene expression of toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear transcription factor κB(NF-κB),NF-κB inhibitory pro-tein α(IκB-α)were detected by PCR.The expression levels of TLR4,MyD88,NF-κBp65 and p-NF-κBp65 in skin lesions were detected by Western Blot.RE-SULTS:Compared with blank group,the lesion score of mice in model group was increased(P<0.01).The epidermis was hyperkeratosis,the spinous layer was hypertrophic,and eosinophils and other inflamma-tory cells infiltrated the dermis.Spleen index and se-rum IgE,IL-6,IL-1β,TNF-alpha and IFN-γ were in-creased(P<0.01),while TGF-β1 was decreased(P<0.01).The expressions of TLR4,MyD88,NF-κB,NF-κBp65 and P-NF-κBP65 were increased,while the ex-pressions of IκB-α were decreased,with statistical significance(P<0.01).Compared with the model group,the skin area of mice in the allergic decoction group was reduced and the score was decreased(P<0.01).The epidermal hyperkeratosis was alleviated,the spinous layer was slightly hypertrophic,and there was a small amount of inflammatory cell infil-tration in the dermis.Spleen index and serum IgE,IL-6,IL-1β,TNF-alpha and IFN-γ were decreased(P<0.01),while TGF-β1 was increased(P<0.01).The ex-pressions of TLR4,MyD88,NF-κB,NF-κBp65 and P-NF-κBP65 were decreased,while the expressions of IκB-α were increased(P<0.05).CONCLUSION:Guomin decoction can relieve pruritus,spleen index and skin inflammation in AD mice.It may regulate in-flammatory response and transcription through TLR signaling pathway,inhibit the activation of p65 sub-unit in NF-κB,and affect the release of downstream inflammatory factors to achieve therapeutic effects.关键词
过敏煎/特应性皮炎/Toll样受体4/髓样分化因子88/核转录因子κB信号通路/动物模型Key words
Guomin decoction/atopic dermatitis/Toll-like receptor 4/myeloid differentiation factor 88/nuclear transcription factor κB signaling pathway activity/animal model分类
医药卫生引用本文复制引用
许琳琳,张豆,丁朋涛,席晓霞,杨鹏斐,张潇亚,李廷保..基于TLR4/MyD88/NF-κB信号通路探讨过敏煎对特应性皮炎小鼠的保护机制[J].中国临床药理学与治疗学,2025,30(12):1648-1657,10.基金项目
甘肃省自然科学基金项目(23JRRA1722) （23JRRA1722）
甘肃省中医药研究中心开放课题资助项目(zyzx-2024-zx08) （zyzx-2024-zx08）
甘肃省自然科学实验动物专项(23JRRA1169) （23JRRA1169）
