中国临床药理学与治疗学2025，Vol.30Issue(12)：1658-1668,11.DOI:10.12092/j.issn.1009-2501.2025.12.009
基于转录组学探讨白连降浊方治疗脂质代谢异常的作用机理
Exploring the mechanism of Bai-lian turbidity reducing formula in treating lipid metabolism disorders based on transcriptomics
摘要
Abstract
AIM:To investigate the effect of C.albi-do on mice with abnormal lipid metabolism induced by high-fat diet based on transcriptomics.METH-ODS:Fifty male SD rats were selected,with 8 desig-nated as the blank group.The remaining 42 rats were fed a high-fat diet(HFD)for model establish-ment.After 4 weeks of modeling,blood samples were taken from the orbital sinus to measure chang-es in serum total cholesterol(TC)and triglyceride(TG)levels to assess the success of the lipid metabo-lism disorder model.Rats modeling failures were ex-cluded,and the 32 successful rats were randomly di-vided into four groups:a positive drug group,a mod-el group,a low-dose Bai Lian Jiang Zhuo Fang group(BLJZ-BD),and a high-dose Bai Lian Jiang Zhuo Fang group(BLJZ-BG),with 8 rats in each group.The posi-tive drug group was treated with atorvastatin calci-um tablets(Avt)at a dosage of 1.25 mg/kg via ga-vage,while the low-dose Bai Lian Jiang Zhuo Fang group was administered 5 g/kg by gavage,and the high-dose group received 10 g/kg by gavage.After 4 weeks of continuous administration,Oil Red O stain-ing was utilized to detect lipid deposition in the liver tissue.Subsequent biochemical indicators were as-sessed,measuring TC,TG,HDL-C,LDL-C,and the lev-els of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in liver tissue.Serum sam-ples were taken to analyze levels of free fatty acids(FFA),lipoprotein lipase(LPL),hepatic triglyceride li-pase(HL),and hormone-sensitive lipase(HSL)in each rat group.Additionally,RNA sequencing tech-nology was employed on rat liver tissue to deter-mine the mRNA abundance of different organisms and further screen significantly changed differential-ly expressed genes,followed by Western blotting to verify the expression of proteins in related signaling pathways.RESULTS:The results of oil red O staining showed that the area of lipid deposition in the liver of the model group was significantly higher than that of the blank group(P<0.01),and the proportion of lipid droplets in the positive drug group and the turbidity reduction group was significantly lower than that in the model group(P<0.01).The results of biochemical index analysis showed that compared with the model group,the positive drug group and the high-dose group of Bailian Turbidity Fang could significantly reduce the levels of TC,TG,LDL-C,ALT and AST in the liver tissue of rats with abnormal lipid metabolism(P<0.01),increase the levels of HDL-C(P<0.01),significantly reduce the levels of FFA and HSL in the serum of rats with abnormal lipid metabolism(P<0.01),and significantly increase the levels of LPL and HL(P<0.05,P<0.01).Transcriptomic studies showed that there were 48 differential metabolites and 288 core callback genes at the intersection of diseases and drugs,and the enrichment pathways were mainly concentrated in PPAR and AMPK path-ways.Western blot verification results showed that the expression of PPARα,CYP7A1 and CPT1A pro-teins could be up-regulated.CONCLUSION:The anti-turbidity formula can improve the abnormal lipid metabolism,and promote the β oxidation of fatty ac-ids and the metabolism of bile acids through the PPAR pathway,there by promoting the lipid metabo-lism process and playing a therapeutic role.关键词
脂质代谢异常/白连降浊方/转录组学/作用机理Key words
abnormal lipid metabolism/Bai Lian Turbidity Reducing formula/transcriptomics/mech-anism of action分类
医药卫生引用本文复制引用
孙嘉宁,王瑞麒,杨卫东,张潞,王艳,程艳刚,王颖莉,郝旭亮..基于转录组学探讨白连降浊方治疗脂质代谢异常的作用机理[J].中国临床药理学与治疗学,2025,30(12):1658-1668,11.基金项目
山西省重点研发计划项目(202202130501012) （202202130501012）
山西省技术创新中心项目(202104010911024) （202104010911024）
山西省科技创新人才团队项目(重点)(202204051002028) （重点）
