中国兽医协会兽医实验室检测分会 1王少林 2田克恭3
作者信息
- 2. 中国农业大学
- 3. 国家兽用药品工程技术研究中心
- 折叠
摘要
Abstract
Targeted next-generation sequencing(tNGS)-based diagnostic technologies enable the rapid acquisition of pathogen sequence information from samples,allowing for the detection and identification,source tracing and genotyping,and comprehensive characterization of animal pathogenic microorganisms,including certain zoonotic pathogens.Therefore,the development of this technology serves not only as a valuable complement to existing nucleic acid-based diagnostic methods for animal diseases,but also as an important technical safeguard for veterinary public health security and the sustained,stable development of the livestock industry.Given that a unified and standardized consensus has not yet been established within the veterinary diagnostic field,this consensus document systematically addresses the application scenarios of tNGS in animal pathogen detection,basic laboratory requirements,guiding principles for primer panel design,experimental workflows,data analysis,result description and interpretation,and sequencing reports,and proposes standardized requirements and practical recommendations.关键词
动物病原微生物/高通量靶向测序(tNGS)/扩增法/专家共识/规范建议Key words
animal pathogenic microorganisms/targeted next-generation sequencing(tNGS)/amplification method/expert consensus/standardization recommendation分类
农业科技
